Cov khoom

Lub Tet 5-methylcytosine dioxygenases catalyze DNA demethylation los ntawm kev tsim 5-hydroxymethylcytosine thiab ntxiv oxidized khoom.Tet1 thiab Tet2 tau qhia ntau heev hauv nas pluripotent hlwb thiab downregulated rau ntau yam hauv somatic hlwb, tab sis transcriptional mechanisms tsis meej.Ntawm no peb tau txhais cov kev txhawb nqa thiab kev txhawb nqa hauv Tet1 thiab Tet2.Tsis pub dhau 15-kb "superenhancer" ntawm Tet1, muaj ob qhov chaw pib hloov pauv (TSSs) nrog cov qauv ua kom sib txawv thaum lub sijhawm txhim kho.Ib cheeb tsam 6-kb txhawb nqa cov dej ntws ntawm qhov distal TSS yog cov nquag nquag hauv cov hlwb tsis muaj pluripotent, autonomously qhia Tet1 qhia nyob rau hauv ib tug transgenic system, thiab sai heev undergoes DNA methylation thiab ntsiag to raws li qhov sib txawv nyob rau hauv kab lis kev cai hlwb thiab haiv neeg epiblast.TSS thib ob nqes dej, cuam tshuam nrog kev txhawb nqa tsis muaj zog CpG-nplua nuj, yog qhib los ntawm cov neeg nyob sib ze ua kom zoo dua hauv cov kab mob tsis zoo embryonic qia hlwb (ESCs) thiab primed epiblast-zoo li hlwb (EpiLCs).Tet2 muaj CpG kob p romoter nrog pluripotency-kev ywj pheej ua si thiab ib qho ESC-tshwj xeeb distal intragenic enhancer;Cov yav tas yog downregulated sai heev nyob rau hauv EpiLCs.Peb txoj kev tshawb fawb nthuav tawm cov qauv sib txawv ntawm kev tswj hwm kev hloov pauv ntawm Tet1 thiab Tet2 thaum lub sijhawm hloov pauv ntawm lub xeev ntawm kev loj hlob ntxov.Cov neeg sau xov xwm hloov pauv tshiab siv Tet1 thiab Tet2 cis-kev tswj hwm cov thawj coj tuaj yeem ua haujlwm kom paub qhov txawv ntawm cov kev hloov pauv hauv cov xeev pluripotent thiab cov kev hloov pauv hauv epigenetic.