Cov khoom

Cov ntaub ntawv pov thawj tsis ntev los no txhawb nqa lub luag haujlwm tseem ceeb ntawm β-nicotinamide adenine dinucleotide (β-NAD(+) ) ua ib qho tshiab neurotransmitter thiab neuromodulator nyob rau hauv lub paj hlwb peripheral -β-NAD(+) raug tso tawm hauv paj hlwb-nqus cov leeg npaj thiab adrenal chromaffin hlwb. nyob rau hauv ib tug yam ntxwv yam ntxwv ntawm ib tug neurotransmitter.Tam sim no tsis paub meej tias qhov no puas muaj tseeb rau CNS.Siv cov kev ntsuam xyuas me me thiab cov kev ntsuas siab siab siab siab ua kua chromatography cov tswv yim, peb pom tau tias high-K(+) stimulation ntawm nas forebrain synaptosomes evokes overflow ntawm β-NAD(+), adenosine 5'-triphosphate, thiab lawv. metabolites adenosine 5'-diphosphate (ADP), adenosine 5'-monophosphate, adenosine, ADP-ribose (ADPR) thiab cyclic ADPR.Lub high-K (+) -evoked overflow ntawm β-NAD (+) yog attenuated los ntawm cleavage ntawm SNAP-25 nrog botulinum neurotoxin A, los ntawm inhibition ntawm N-hom voltage-dependent Ca(2+) raws nrog ω-conotoxin GVIA , thiab los ntawm inhibition ntawm proton gradient ntawm synaptic vesicles nrog bafilo mycin A1, tawm tswv yim tias β-NAD (+) zoo li tso tawm ntawm vesicle exocytosis.Kev soj ntsuam sab hnub poob pom tau tias CD38, ntau cov protein ntau uas metabolizes β-NAD (+), muaj nyob rau ntawm cov ntaub so ntswg synaptosomal thiab hauv cytosol.Intact synaptosomes degrade β-NAD(+).1, N (6) -etheno-NAD, ib qho fluorescent analog ntawm β-NAD (+), yog coj los ntawm synaptosomes thiab qhov no uptake yog attenuated los ntawm cov qhabnias β-NAD(+), tab sis tsis yog los ntawm connexin 43 inhibitor Gap 27. Nyob rau hauv cortical neurons hauv zos daim ntaub ntawv ntawm β-NAD(+) ua rau Ca(2+) transients sai, tej zaum vim influx ntawm extracellular Ca(2+).Yog li ntawd, nas hlwb synaptosomes tuaj yeem tso tawm, txo qis thiab nce β-NAD(+), thiab β-NAD(+) tuaj yeem txhawb nqa postsynaptic neurons, txhua yam xav tau rau cov khoom siv los txiav txim siab tus neeg sib tw neurotransmitter hauv lub hlwb.