Cov khoom

Infrared spectroscopy tau siv los ua daim ntawv qhia kev sib cuam tshuam hauv substrate-protein: kev hloov pauv ntawm sarcoplasmic reticulum Ca(2+)-ATPase raws li nucleotide khi thiab ATPase phosphorylation tau saib xyuas siv cov substrate ATP thiab ATP analogues (2'-deoxy-ATP, '-deoxy-ATP, thiab inosine 5'-triphosphate), uas tau hloov kho ntawm cov haujlwm tshwj xeeb ntawm cov substrate.Kev hloov kho rau 2'-OH, 3'-OH, thiab cov amino pawg ntawm adenine txo qhov cuam tshuam ntawm kev sib txuas-induced conformational hloov ntawm ATPase, nrog rau tshwj xeeb muaj zog tshwm sim rau ob tom kawg.Qhov no qhia tau hais tias cov txheej txheem rhiab heev ntawm nucleotide-ATPase complex rau tus kheej kev sib cuam tshuam ntawm nucleotide thiab ATPase.Txhua pab pawg tau kawm yog qhov tseem ceeb rau kev khi thiab kev sib cuam tshuam ntawm ib pab pawg ligand nrog ATPase nyob ntawm kev sib cuam tshuam ntawm lwm pawg ligand.Phosphorylation ntawm ATPase tau soj ntsuam rau ITP thiab 2'-deoxy-ATP, tab sis tsis yog rau 3'-deoxy-ATP.Tsis muaj qhov sib txuas ncaj qha ntawm nws qhov kev hloov pauv raws li nucleotide khi thiab tus nqi ntawm phosphorylation uas qhia tias tag nrho cov kev hloov pauv ntawm ATP-induced conformational tsis yog yuav tsum tau rau phosphorylation.Raws li pom rau nucleotide-ATPase complex, qhov ua raws ntawm thawj phosphorylated ATPase nruab nrab E1PCa(2) kuj nyob ntawm nucleotide, qhia tias ATPase xeev muaj qhov tsis sib xws tsawg dua li yav tas los xav.