Cov khoom

Peb yav dhau los tau ntsib cov txheej txheem tswj hwm qhov twg dihydrotestosterone (DHT) tau ua rau nws cov nyhuv inhibitory ntawm parathyroid hormone-related protein (PTHrP) gene repression los ntawm cov tshuaj estrogen receptor (ER)α, tab sis tsis yog androgen receptor (AR), nyob rau hauv mob cancer mis MCF-7 hlwb. .Ntawm no, peb tau tshawb xyuas seb puas muaj kev sib cuam tshuam ligand-nuclear receptor (NR) hauv prostate cancer LNCaP hlwb.Ua ntej, peb tau lees paub tias LNCaP hlwb tau nthuav tawm ntau ntawm AR ntawm qib tsis pom zoo ntawm ERα / β lossis progesterone receptor.Ob qho kev tawm tsam ntawm PTHrP thiab kev ua kom cov prostate-specific antigen noob tau pom tom qab kev tswj hwm ywj pheej ntawm 17β-estradiol (E2), DHT, lossis R5020.Raws li qhov kev xav tias LNCaP AR poob nws qhov ligand tshwj xeeb vim muaj kev hloov pauv (Thr-Ala877), kev sim nrog siRNA lub hom phiaj ntawm NR tau qhia tias AR monopolized lub luag haujlwm ntawm tus neeg kho kom haum xeeb ntawm kev sib koom cov tshuaj hormone-dependent kev cai, uas yog invariably txuam. nrog nuclear translocatio n ntawm no mutant AR.Microarray tsom xam ntawm cov noob kev tswj hwm los ntawm DHT, E2, lossis R5020 tau tshaj tawm tias ntau tshaj li ib nrab ntawm cov noob hauv qab ntawm AR (Thr-Ala877) sib tshooj hauv LNCaP hlwb.Ntawm qhov kev txaus siab tshwj xeeb, peb pom tau hais tias AR (wt-type [wt]) thiab AR (Thr-Ala877) yog tib lub luag haujlwm rau E2-AR kev sib cuam tshuam.Fluorescence microscopy thwmsim pom tau tias ob qho tib si EGFP-AR (wt) thiab EGFP-AR (Thr-Ala877) tau tshwj xeeb hauv cheeb tsam hauv lub nucleus tom qab E2 lossis DHT kho.Tsis tas li ntawd, cov kws tshuaj ntsuam xyuas tau tshaj tawm tias qee cov qog nqaij hlav qog noj ntshav tau nthuav tawm qhov tsis txaus ntseeg E2-AR (wt) cov cim qhia zoo ib yam li hauv LNCaP hlwb.Peb ntawm no qhia txog qhov muaj kev sib cuam tshuam ntawm wt AR thiab E2 hauv qee cov qog nqaij hlav qog nqaij hlav qog nqaij hlav.