D-Luciferin Cas: 2591-17-5 99% Tawm-dawb rau daj hmoov nbsp BEETLE LUCIFERIN
| Catalog Number | xwm 90248 |
| Khoom npe | D-Luciferin |
| CAS | 2591-17-5 ib |
| Molecular Formula | C11H8N2O3S2 |
| Molecular Luj | 280.323 ib |
| Cov ntaub ntawv khaws cia | -15 rau -20 ° C |
| Harmonized tariff Code | 29342080 ib |
Khoom Specification
| Cov ntsiab lus dej | Max.2.0% |
| Tawg | Max 2.0 NTU |
| Kev sib hloov kho qhov muag tshwj xeeb | -36-32 |
| Qhov tshwm sim | Tawm-dawb mus rau daj hmoov |
| Purity HPLC | Tsawg kawg 99% |
| Molar extinction coefficient | Min 17900 L / (mol cm) |
Taw qhia: D-luciferin yog substrate rau adenosine triphosphate (ATP)-dependent bioluminescence cov tshuaj tiv thaiv.Lub hauv paus ntsiab lus ntawm bioluminescence yog tias luciferin yog oxidized los ntawm luciferase nyob rau hauv lub xub ntiag ntawm ATP thiab oxygen.Cov tshuaj tiv thaiv mis yog raws li nram no: ATP + D-Luciferin + O2 → Oxyluciferin + AMP + PPi + O2 + Teeb.
Mechanism ntawm kev txiav txim: Lub mechanism ntawm kev txiav txim ntawm D-luciferin yog hais tias nyob rau hauv qhov kev txiav txim ntawm ATP thiab luciferase, luciferin (substrate) yuav oxidized kom emit lub teeb.Thaum luciferin ntau dhau, tus naj npawb ntawm photons tsim tau zoo sib raug zoo nrog cov concentration ntawm luciferase.
Daim ntawv thov: D-luciferin yog substrate rau adenosine triphosphate (ATP)-nyob ntawm bioluminescence cov tshuaj tiv thaiv.Cov tshuaj bioluminescence ntawm luciferin / luciferase feem ntau yog siv rau kev tshawb pom ntawm ATP, metabolites uas tuaj yeem hloov mus rau ATP (xws li AMP, ADP, cAMP), thiab cov enzymes uas tuaj yeem tsim ATP (xws li creatine kinase, thiab lwm yam), yog li. Cov tshuaj tiv thaiv bioluminescence tuaj yeem siv tau.Muaj feem xyuam rau kev tshawb nrhiav ntau yam khoom siv lom neeg.
Kev Ua Txhaum Cai: D-Luciferin (Firefly luciferin) yog qhov nrov substrate rau bioluminescence nyob rau hauv lub xub ntiag ntawm ATP rau siv nyob rau hauv luciferase-based bioluminescence imaging thiab cell-based high-throughput screening.
Kev tshawb fawb hauv vitro: D-luciferin reacts nrog luciferase, ATP thiab oxygen kom emit lub teeb, uas yog kuaj pom los ntawm rhiab duab zaj duab xis kom pom alkaline phosphatase-bound antibodies.
Hauv vivo cov kev tshawb fawb: kev siv D-luciferin substrates thiab firefly luciferase khaws cov qog-tus tswv lub cev tiv thaiv kev sib cuam tshuam hauv tus qauv immunocompetent nas nrog mob qog noj ntshav ntawm zes qe menyuam, raws li cov kev pabcuam bioluminescence muaj kev nkag siab rau cov qog loj dua li cov lus qhia ntawm lub cev hnyav.
