Cov khoom

Taw qhia: BSA yog ib qho ntawm cov protein uas siv ntau tshaj plaws hauv cov chaw sim tshuaj biochemical, thiab nws qhov tseem ceeb yuav raug saib xyuas hauv kev sim vim tias nws muaj ntau dhau thiab ntau dhau lawm.Bovine serum albumin (BSA), tseem hu ua lub thib tsib tivthaiv, yog ib tug globulin nyob rau hauv bovine serum muaj 583 amino acid residues, nrog ib tug molecular hnyav ntawm 66.430kDa thiab ib tug isoelectric point ntawm 4.7.BSA muaj ntau yam kev siv hauv kev sim tshuaj biochemical, xws li Blocking agent nyob rau sab hnub poob blots.

Daim ntawv thov: Bovine Serum Albumin (BSA), tseem hu ua lub thib tsib tivthaiv, yog globulin nyob rau hauv bovine serum, muaj 607 amino acid residues, thiab muaj ntau yam kev siv nyob rau hauv biochemical thwmsim.Bovine serum albumin feem ntau yog siv los ua tus stabilizer nyob rau hauv cov kev daws teeb meem thiab cov tshuaj tiv thaiv ntawm kev txwv enzymes los yog hloov enzymes kom stabilize enzyme kev ua si thiab tiv thaiv enzyme decomposition thiab non-specific adsorption.

Kev Ua Haujlwm: BSA feem ntau yog siv los ua lub zog ruaj khov hauv cov kev daws teeb meem thiab cov tshuaj tiv thaiv ntawm kev txwv cov enzymes lossis hloov cov enzymes, vim qee cov enzymes tsis ruaj khov lossis muaj kev ua haujlwm tsawg ntawm cov concentrations tsawg.Tom qab ntxiv BSA, nws tuaj yeem ua lub luag haujlwm ntawm "kev tiv thaiv" lossis "tus neeg nqa khoom", thiab kev ua haujlwm ntawm ntau cov enzymes tuaj yeem txhim kho tau zoo tom qab ntxiv BSA.Enzymes uas tsis tas yuav ntxiv BSA feem ntau tsis cuam tshuam los ntawm kev ntxiv BSA.Rau feem ntau cov substrate DNA, BSCA tuaj yeem ua rau kev zom zaub mov kom tiav, thiab tuaj yeem ua tiav kev txiav rov qab.Ntawm 37 ° C, thaum cov tshuaj tiv thaiv kev zom zaub mov ntau dua 1 h, BSA tuaj yeem ua rau cov enzymes ruaj khov, vim tias nyob rau hauv cov tshuaj tiv thaiv tsis muaj BSA, ntau cov enzymes txwv tsis pub tsuas yog muaj sia nyob ntawm 37 ° C rau 10 ~ 20min lossis lub sijhawm luv dua..Hauv qhov sib piv, BSA tuaj yeem khi hlau ions thiab lwm yam tshuaj nyob rau hauv tsis los yog substrate DNA uas inhibit qhov kev ua ntawm kev txwv endonucleases.

Siv: Standard grade bovine serum albumin (BSA, StandardGrade), uas tuaj yeem ua tau raws li qhov xav tau ntawm feem ntau cov kev sim niaj hnub, xws li cov tshuaj tiv thaiv kab mob, cov ntaub so ntswg cell (microbial tsiaj thiab kab kab, thiab lwm yam) kab lis kev cai as-ham thiab kab lis kev cai Cheebtsam, protein / enzyme stabilization reagents thiab protein ntau cov qauv.Diagnostic grade bovine serum albumin (BSA, DiagnosticGrade) tuaj yeem ua tau raws li cov kev xav tau niaj hnub ua, xws li cov tshuaj tiv thaiv kab mob, cov protein / enzyme stabilizer, diluent, carrier thiab protein standard.Tsis tas li ntawd, nws tseem tuaj yeem siv rau immunoassays, kab lis kev cai ntawm tes thiab hybridization thwmsim uas xav tau siab rhiab heev.