Cov khoom

Lub brucellae yog cov kab mob etiological ntawm brucellosis, ib qho kev faib thoob ntiaj teb zoonosis.Cov kab mob no yog cov kab mob facultative intracellular parasites thiab yog li muaj peev xwm kho lawv cov metabolism hauv qhov chaw ntxiv- thiab intracellular ib puag ncig ntsib thaum lub sijhawm sib kis.Txawm li cas los xij, qhov no ntawm Brucella biology yog qhov tsis paub meej, thiab cov as-ham muaj nyob rau hauv intracellular niche tsis paub.Ntawm no, peb tshawb xyuas txoj hauv kev hauv nruab nrab ntawm C metabolism siv los ntawm Brucella abortus los ntawm kev tshem tawm cov fructose-1,6-bisphosphatase (fbp thiab glpX), phosphoenolpyruvate carboxykinase (pckA), pyruvate phosphate dikinase (ppdK), thiab malic enzyme (mae) noob.Hauv gluconeogenic tab sis tsis nyob hauv cov xov xwm nplua nuj, kev loj hlob ntawm ΔppdK thiab Δmae mutants tau raug mob hnyav thiab kev loj hlob ntawm ob Δfbp-ΔglpX mutant tau txo.Hauv macrophages, tsuas yog ΔppdK thiab Δmae mutants pom qhov txo qis, thiab kev tshawb fawb nrog ΔppdK mutant tau lees paub tias nws mus txog qhov sib txawv ntawm cov niche.Ib yam li ntawd, cov ΔppdK thiab Δmae mutants tau attenuated nyob rau hauv nas, yav dhau los raug tshem tawm los ntawm lub lim tiam 10 thiab tom kawg txuas ntxiv ntev dua 12 lub lis piam.Peb kuj tau tshawb xyuas lub voj voog glyoxylate.Txawm hais tias aceA (isocitrate lyase) kev txhawb nqa kev ua haujlwm tau txhim kho hauv qhov nruab nrab nplua nuj, aceA cuam tshuam tsis muaj kev cuam tshuam hauv vitro lossis kev sib faib hauv macrophages lossis nas spleens.Cov txiaj ntsig tau qhia tias B. abortus loj hlob intracellularly siv cov khoom siv tsawg ntawm 6-C (thiab 5-C) cov suab thaj uas tau them los ntawm glutamate thiab tej zaum lwm cov amino acids nkag mus rau lub voj voog Krebs yam tsis muaj lub luag haujlwm tseem ceeb ntawm glyoxylate shunt.