Biosynthesis ntawm proteoglycans thiab glycosaminoglycans nyob rau hauv lub xub ntiag ntawm p-nitrophenyl-xyloside tau kawm siv ib tug thawj nas ntawm zes qe menyuam granulosa cell kab lis kev cai system.Ntxiv ntawm p-nitrophenyl-xyloside rau hauv cell kab lis kev cai nruab nrab ua rau kwv yees li 700% nce ntawm [35S] sulfate incorporation (ED50 ntawm 0.03 mM) rau hauv macromolecules, uas suav nrog dawb chondroitin sulfate chains pib ntawm xyloside thiab haiv neeg proteoglycans.Dawb chondroitin sulfate chains pib ntawm xyloside yuav luag tshwj xeeb zais rau hauv nruab nrab.Cov molecular loj ntawm chondroitin sulfate chains poob los ntawm 40,000 mus rau 21,000 raws li tag nrho [35S] sulfate incorporation tau txhim kho, qhia tias txhim kho synthesis ntawm chondroitin sulfate perturbed lub cev mechanism ntawm glycosaminoglycan saw txiav.Biosynthesis ntawm heparan sulfate proteoglycans raug txo los ntawm kwv yees li 50%, yuav yog vim muaj kev sib tw ntawm qib UDP-qab zib precursors.[35S] Sulfate incorporation raug kaw los ntawm kev sib ntxiv ntawm cycloheximide nrog thawj ib nrab sij hawm ntawm kwv yees li 2 hr nyob rau hauv lub xub ntiag ntawm xyloside, thaum uas tsis muaj xyloside yog li 20 min.Qhov sib txawv yuav qhia txog qhov kev hloov pauv ntawm glycosaminoglycan synthesizing muaj peev xwm tag nrho.Kev hloov pauv ntawm glycosaminoglycan synthesizing muaj peev xwm pom nyob rau hauv zes qe menyuam granulosa hlwb yog luv dua li uas tau pom nyob rau hauv chondrocytes, xav txog tus txheeb ze dominance ntawm proteoglycan biosynthetic kev ua si nyob rau hauv tag nrho cov metabolic kev ua ntawm lub hlwb.
