X-GAL CAS: 7240-90-6 98% Dawb rau dawb-dawb Crystalline hmoov
| Catalog Number | XD90008 |
| Khoom npe | X-Gal (5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) |
| CAS | 7240-90-6 : kuv |
| Molecular Formula | C14H15BrClNO6 |
| Molecular Luj | 408.63 ib |
| Cov ntaub ntawv khaws cia | -2 txog -6 ° C |
| Harmonized tariff Code | 29 400 000 IB |
Khoom Specification
| Qhov tshwm sim ntawm kev daws teeb meem | Ntshiab, tsis muaj xim rau lub teeb daj (50mg / ml hauv DMF: MeOH, 1: 1) |
| Kev sib hloov kho qhov muag tshwj xeeb | -61.5 +/- 1 |
| Qhov tshwm sim | Dawb mus rau dawb crystalline hmoov |
| Purity HPLC | tsawg kawg 99% |
| Solubility (5% hauv DMF) | Soluble (5% w/v, DMF) |
| KF dej | max 1% |
| Assay (HPLC ntawm Anhydrous Basis) | min 98% w/w |
Kev siv X-gal
X-gal (tseem hu ua BCIG rau 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) yog cov organic compound uas muaj galactose txuas mus rau qhov hloov pauv indole.Lub compound tau tsim los ntawm Jerome Horwitz thiab cov neeg koom tes hauv xyoo 1964.Lub npe tshuaj raug cai feem ntau luv luv kom tsis muaj tseeb tab sis kuj tsis tshua muaj cov kab lus xws li bromochloroindoxyl galactoside.X los ntawm indoxyl tuaj yeem yog qhov chaw ntawm X hauv X-gal contraction.X-gal feem ntau yog siv hauv molecular biology los ntsuas qhov muaj enzyme, β-galactosidase, nyob rau hauv qhov chaw ntawm nws lub hom phiaj ib txwm, β-galactoside.Nws kuj tseem siv los txheeb xyuas cov haujlwm ntawm cov enzyme no hauv histochemistry thiab bacteriology.X-gal yog ib qho ntawm ntau indoxyl glycosides thiab esters uas tawm los ntawm cov xim xiav tsis sib haum zoo ib yam li indigo dye vim los ntawm enzyme-catalyzed hydrolysis.
X-gal yog ib qho analog ntawm lactose, thiab yog li ntawd tej zaum yuav hydrolyzed los ntawm β-galactosidase enzyme uas cleaves β-glycosidic daim ntawv cog lus nyob rau hauv D-lactose.X-gal, thaum cleaved los ntawm β-galactosidase, yields galactose thiab 5-bromo- 4-chloro-3-hydroxyindole - 1. Tom qab ntawd spontaneously dimerizes thiab yog oxidized rau hauv 5,5'-dibromo-4,4'-dichloro -indigo - 2, ib qho khoom siv xiav heev uas yog insoluble.X-gal nws tus kheej tsis muaj xim, yog li cov khoom muaj xim xiav tuaj yeem siv los ua qhov kev sim rau qhov muaj β-galactosidase.Qhov no kuj tso cai rau cov kab mob β-galactosidase (hu ua lacZ) los ua tus tshaj tawm hauv ntau daim ntawv thov.
Hauv kev tsom xam ob-hybrid, β-galactosidase tuaj yeem siv los ua tus tshaj tawm los txheeb xyuas cov proteins uas cuam tshuam nrog ib leeg.Hauv cov qauv no, cov tsev qiv ntawv genome yuav raug tshuaj xyuas rau cov protein sib cuam tshuam siv cov poov xab lossis kab mob.Qhov twg muaj kev sib raug zoo ntawm cov proteins uas raug tshuaj xyuas, nws yuav ua rau muaj kev cuam tshuam ntawm kev ua kom muaj zog rau tus txhawb nqa.Yog tias tus txhawb nqa txuas nrog lacZ gene, kev tsim cov β-galactosidase, uas ua rau tsim cov xim xiav-pigmented nyob rau hauv lub xub ntiag ntawm X-gal, yuav qhia tau tias muaj kev sib raug zoo ntawm cov proteins.Cov txheej txheem no yuav raug txwv rau kev tshuaj xyuas cov tsev qiv ntawv ntawm qhov loj me ntawm qhov tsawg dua li ntawm 106.Qhov kev vam meej ntawm X-gal kuj tsim cov ntxhiab tsw ntxhiab tsw zoo vim lub volatilization ntawm indole.
Raws li X-gal nws tus kheej tsis muaj xim, lub xub ntiag ntawm cov khoom xiav-xim tuaj yeem siv los ua qhov kev sim rau qhov muaj β-galactosidase.
Qhov kev txheeb xyuas yooj yim ntawm cov enzyme nquag tso cai rau cov noob rau βgalactosidase (lub lacZ gene) siv los ua tus neeg sau xov xwm gene hauv ntau daim ntawv thov.
