Cov khoom

Biotransformation ntawm methyl-β-D-glucopyranoside rau siab dua cov saw alkyl glucosides los ntawm cell bound β-glucosidase ntawm Pichia etchellsii.

Hauv txoj kev tshawb no, peb tau tshawb xyuas kev siv Pichia etchellsii tag nrho cov hlwb rau kev sib txuas ntawm cov saw ntev alkyl glucosides.Methyl-β-d-glucopyranoside (MG) tau siv rau hauv cov tshuaj tiv thaiv nrog fatty alcohols, n-hexanol, n-octanol, n-decanol thiab n-dodecanol los ua ke cov alkyl glucosides.Thawj cov tshuaj tiv thaiv thawj zaug tau ua kom zoo dua ntawm 2.5 ml nplai rau kev sib txuas ntawm octyl glucoside (OG) thiab yog 8% cov ntsiab lus dej, 100mM MG thiab 6h ntawm cov tshuaj tiv thaiv lub sijhawm thiab qhov no ua rau ≈ 53% tawm los.Qhov siab tshaj plaws transglucosylation / hydrolysis piv ntawm 2.79 tau txais ntawm 100mM MG nyiam cov khoom lag luam siab.Raws li qhov ua tau zoo, lub reactor tau ua haujlwm ntawm 50 ml theem uas ua rau ≈ 60% hloov pauv ntawm MG rau OG.Ib txoj kev ua kua chromatography yooj yim yog tsim los rau kom muaj nuj nqis ntawm cov saw glucosides siab dua siv lub ntsuas ntsuas qhov ntsuas qhov ntsuas.Qhov siab tshaj plaws ntawm 27% thiab 13% tawm los rau decyl-, thiab dodecyl-β-d-glucopyranoside, raws li