Cov kab mob entomopathogenic, Xenorhabdus nematophila, induces ib qho kev tiv thaiv kab mob ntawm cov kab mob los ntawm inhibiting phospholipase A(2) (PLA(2)) kev ua.Tsis ntev los no, ib qho kev tiv thaiv kab mob sib txuas nrog PLA(2) noob tau txheeb xyuas los ntawm cov hmoov nplej liab, Tribolium castaneum.Txoj kev tshawb no cloned no PLA(2) noob nyob rau hauv ib tug kab mob qhia vector los tsim ib tug recombinant enzyme.Lub recombinant T. castaneum PLA(2) (TcPLA(2)) nthuav tawm nws cov yam ntxwv enzyme kev ua haujlwm nrog substrate concentration, pH, thiab ambient kub.Nws cov yam ntxwv biochemical matched rau ib hom secretory ntawm PLA(2) (sPLA(2)) vim hais tias nws cov dej num yog inhibited los ntawm dithiothreitol (ib tug txo tus neeg sawv cev ntawm disulfide daim ntawv cog lus) thiab bromophenacyl bromide (ib qho tshwj xeeb sPLA(2) inhibitor) tab sis tsis yog los ntawm methylarachidonyl. fluorophosphonate (ib hom cytosolic tshwj xeeb ntawm PLA(2)).X. nematophila kab lis kev cai broth muaj PLA(2) inhibitory yam (s), uas yog ntau tshaj nyob rau hauv cov xov xwm tau txais nyob rau hauv ib tug nyob ruaj ruaj kab mob loj hlob theem.PLA(2) inhibitory yam (s) yog kub-resistant thiab muab rho tawm nyob rau hauv ob qho tib si aqueous thiab organic feem.Cov nyhuv ntawm PLA(2)-inhibitory feem ntawm kev tiv thaiv kab mob ntawm T. castaneum tau sib npaug sib piv nrog qhov tshwm sim los ntawm inhibition ntawm TcPLA(2) noob qhia los ntawm RNA cuam tshuam.
