Cov khoom

Acridine ester (NSP-SA-NHS) Cas199293-83-9 thiab nws cov tshuaj muaj feem xyuam yog cov ntawv zoo chemiluminescent uas nws ruaj khov, ua haujlwm thiab rhiab heev tshaj qee cov xov tooj cua.Acridine esters tuaj yeem cuam tshuam nrog cov proteins uas muaj cov amino pawg thawj.Raws li cov xwm txheej yooj yim, NHS tau hloov pauv raws li pab pawg tawm, thiab cov protein ua kom ruaj khov amide nrog acridine ester.Tom qab cov tshuaj tiv thaiv tiav lawm, cov ntsev ntau dhau acridinium raug tshem tawm los ntawm kab desalting.

Acridine-labeled proteins tsis tas yuav enzymatic catalysis kom emit lub teeb nyob rau hauv lub xub ntiag ntawm alkaline hydrogen peroxide.Lub hauv paus ntsiab lus ntawm lub teeb-emitting yog hais tias nyob rau hauv ib tug alkaline hydrogen peroxide tov, acridine ester yog tawm tsam los ntawm hydrogen peroxide ions los tsim ib tug tsis ruaj tsis khov dioxyethane nrog nro, uas yog ntxiv decomposed rau hauv CO2 thiab electronically zoo siab acridone.Thaum lub acridone rov qab mus rau hauv av, nws emits photons nrog lub siab tshaj plaws nqus wavelength ntawm 430 nm.Cov txheej txheem luminescence no luv heev (tag nrho cov txheej txheem yuav siv sij hawm tsawg tshaj li 2 vib nas this), thiab cov txheej txheem triggering yuav tsum ntxiv ib tug sab hauv photometer thiab photon detector;Nyob rau hauv tas li ntawd, cov khoom no kuj siv tau ib tug multi-function microplate nyeem ntawv nruab nrog ib tug autosampler rau luminescence cov ntaub ntawv sau.Proteins, peptides, tshuaj tiv thaiv kab mob, thiab nucleic acids tuaj yeem sau nrog cov khoom no.Acridine esters emit lub teeb sai sai nyob rau hauv lub excitation ntawm alkaline hydrogen peroxide, yog li cov ntawv sau cov tebchaw tuaj yeem kuaj pom los ntawm kev sau cov photons.

Cov khoom no tsuas yog siv rau: chemiluminescence thiab immunoassay, receptor tsom xam, nucleic acid thiab peptide nrhiav thiab lwm yam kev tshawb fawb.