Cov khoom

Kev ntsuas ntawm alpha-amylase nrog 4-nitrophenyl glucosides muab cov txiaj ntsig zoo hauv qab no ntawm cov txheej txheem uas tso siab rau kev tsim NADH: lub sijhawm luv luv, tsis muaj kev cuam tshuam los ntawm cov metabolites thiab enzymes ntawm cov qauv thiab tsis tshua muaj kev ruaj khov substrates nrog cov nqi qis.Lub rhiab heev ntawm kev tsim nitrophenol yog sib npaug ntawm hydrolysis ntawm maltotetraose, tab sis tsawg dua li cov qauv tsim cov piam thaj siv oligosaccharides.Hauv kev sib piv rau cov hmoov txhuv nplej siab, cov chromogenic substrates yog hydrolysed sai dua los ntawm salivary dua li pancreatic amylase.Qhov tsis zoo ntawm cov substrates no yog: cov tsiaj txhu ntau dua los ntawm tib neeg amylases, thiab cov cim ntawm chromophore rau kev hloov me me ntawm pH thiab protein concentration.Qee qhov kev soj ntsuam zoo xws li qhov tshwj xeeb, qhov tseeb, qhov tseeb, kev ruaj ntseg ntawm cov substrate thiab linear ntau yam tau piav qhia meej thiab muab piv nrog rau lwm txoj hauv kev.