Cov khoom

1.Optically nquag nquag D-2-hydroxybutanoate yog ib qho tseem ceeb hauv tsev thaiv nruab nrab rau cov tshuaj thiab biodegradable poly (2-hydroxybutanoate).Kinetic daws teeb meem ntawm racemic 2-hydroxybutanoate tej zaum yuav yog ib qho ntsuab thiab xav tau lwm txoj rau D-2-hydroxybutanoate ntau lawm.Hauv kev ua haujlwm no, D-2-hydroxybutanoate ntawm qhov siab siab (0.197 M) thiab siab enantiomeric tshaj (99.1%) tau tsim los ntawm NAD-ywj siab L-lactate dehydrogenase (L-iLDH) uas muaj biocatalyst.2-Oxobutanoate, lwm qhov tseem ceeb nruab nrab, tau sib koom ua ke ntawm qhov siab siab (0.193 M).Siv cov txheej txheem sib pauv ion yooj yim nrog cov macroporous anion txauv resin D301, D-2-hydroxybutanoate tau cais tawm ntawm biotransformation system nrog kev rov zoo siab ntawm 84.7%.

2.Asymmetric synthesis ntawm unnatural amino acid tau pom los ntawm omega-transaminase los ntawm Vibrio fluvialis JS17.L-2-Aminobutyric acid tau tsim los ntawm 2-oxobutyric acid thiab benzylamine nrog enantiomeric ntau tshaj 99%.Cov tshuaj tiv thaiv tau pom cov khoom lag luam hnyav inhibition los ntawm benzaldehyde, uas tau kov yeej los ntawm kev ua haujlwm biphasic cov tshuaj tiv thaiv kom tshem tawm cov khoom inhibitory los ntawm theem aqueous.Nyob rau hauv ib tug raug biphasic cov tshuaj tiv thaiv (50 mM 2-oxobutyric acid, 70 mM benzylamine thiab 2.64 U / ml purified enzyme) siv hexane raws li ib tug extractant, hloov dua siab tshiab ntawm 2-oxobutyric acid mus txog 96% nyob rau hauv 5 h whereas tsuas 39% hloov dua siab tshiab tau tsis tau. cov khoom extraction.