Cov khoom

Hauv txoj kev kinetic angiotensin-hloov enzyme (ACE) txoj kev, qhov ua tau zoo thiab qhov tsis zoo yog 80 mmol / L borate tsis nyob ntawm pH 8.2 (37 degrees C).Ib theem lag luam raug kuaj pom hauv cov tshuaj tiv thaiv, thiab 5-min incubation ntawm substrate thiab plasma yog pom zoo ua ntej ntsuas kinetic.Lub substrate, N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG), qhov siab tshaj plaws ntawm 1.0 mmol / L thiab ntsuas wavelength yog 345 nm los xyuas kom meej linearity ntawm kev ntsuas.Cov txheej txheem npaj siv 1: 9 plasma-rau-reagent ntim piv.Lub linear ntau ntawm qhov kev ntsuam xyuas txuas mus rau kwv yees li 170 U / L, sawv cev rau 25% substrate hydrolysis.FAPGG absorptivity yog txiav txim siab los ntawm kev ntsuas qhov sib txawv ntawm qhov nqus ntawm 1.0 mmol / L FAPGG thiab cov khoom daws teeb meem.Lub wavelength fidelity yog soj ntsuam los ntawm kev ceeb toom qhov xav tau absorbance tus nqi ntawm FAPGG tov, thiab ib tug 1.0-nm sib txawv ntawm 345 nm hloov lub absorbance los ntawm 15.5%.Qhov tseeb ntawm ACE kev soj ntsuam ntawm kwv yees li 60 thiab 100 U / L yog 3.5% thiab 2.4% hauv batch thiab 2.9% thiab 2.6% ntawm batch, feem.Lub sij hawm siv (2.5 mus rau 97.5 feem pua) yog 41-139 U / L, thiab tsis muaj qhov sib txawv ntawm qhov tseem ceeb rau txiv neej thiab poj niam.