γ-L-Glutamyl-3-carboxy-4-nitroanilide, monoammonium ntsev Cas: 63699-78-5 Lub teeb daj microcrystalline hmoov
| Catalog Number | xwm 90183 |
| Khoom npe | γ-L-Glutamyl-3-carboxy-4-nitroanilide, monoammonium ntsev |
| CAS | 63699-78-5 |
| Molecular Formula | C12H12N3O7-.H4N+ |
| Molecular Luj | 328.27 Nws |
| Cov ntaub ntawv khaws cia | Ambient |
Khoom Specification
| Qhov tshwm sim | Lub teeb daj microcrystalline hmoov |
| Assay | 99% |
| Melting point | 187ºC ± 2.0ºC |
| Boiling point | 718.3 ° C ntawm 760 mmHg |
| Flash point | 368.5°C dej |
| Solubility | H2O: 100 mg/ml, ntshiab, daj-ntsuab |
Cov kinetics ntawm tib neeg cov ntshav gamma-glutamyltransferase (EC 2.3.2.2) tau tshawb xyuas, nrog kev siv glycylglycine ua gamma-glutamyl txais substrate thiab gamma-glutamyl-4-nitroanilide thiab nws cov carboxy derivative, gamma-glutamyl-3-carboxy -nitroanilide, raws li pub dawb substrates.Qhov tshwm sim ib txhij ntawm ob qho tib si gamma-glutamyltransfer thiab autotransfer tau tsim los ntawm cov ntawv sau chromatography.Constant-ratio ob-reciprocal plots paub meej tias lub enzyme mechanism yog nonsequential (ping-pong bi-bi).Inhibition los ntawm ob tus neeg pub dawb tsis pom, thiab inhibition los ntawm glycylglycine tsuas yog pom ntawm qhov siab tshaj ntawm cov kev mob siab rau.Kinetic tas mus li tau los ntawm nonlinear regression tsom xam ntawm thawj cov ntaub ntawv tshaj tawm tau siv los txiav txim siab reagent substrate concentrations rau qhov kev ntsuam xyuas ntawm cov enzyme no.Kev soj ntsuam nrog kev siv 4 mmol ntawm gamma-glutamyl-3-carboxy-4-nitroanilide thiab 100 mmol ntawm glycylglycine ib liter tau muab cov dej num sib npaug rau cov kev soj ntsuam nrog kev siv 4 mmol ntawm gamma-glutamyl-4-nitroanilide thiab 40 mmol. glycylglycine ib liter.Cov concentrations ntawm carboxy pub thiab glycylglycine kuj yog "tus nqi zoo" thiab tsis muaj teeb meem txheej txheem thaum siv.
