Cov khoom

Carbonic anhydrase activators: thawj qhov kev tshawb fawb ntawm tib neeg secretory isoform VI nrog cov amino acids thiab amines.

Lub secretory isozyme ntawm tib neeg carbonic anhydrase (hCA, EC 4.2.1.1), hCA VI, tau cloned, qhia, thiab purified nyob rau hauv ib tug kab mob qhia system.kinetic parameters rau CO(2) hydration cov tshuaj tiv thaiv ua pov thawj hCA VI kom muaj ak(cat) ntawm 3.4 x 10(5)s(-1) thiab k(cat)/K(M) ntawm 4.9 x 10(7)M (-1)s(-1) (ntawm pH 7.5 thiab 20 ° C).hCA VI muaj ib qho tseem ceeb catalytic kev ua si rau lub physiological cov tshuaj tiv thaiv, ntawm tib qhov kev txiav txim ntawm magnitude raws li ubiquitous isoform CA I los yog lub transmembrane, qog-associated isozyme CA IX.Ib qho ntawm cov amino acids thiab amines tau pom tias ua raws li CA VI activators, nrog cov txiaj ntsig sib txawv.l-Nws, l-Trp, thiab dopamine pom tsis muaj zog CA VI ua kom muaj zog (K(A)s nyob rau hauv thaj tsam ntawm 21-42 microM), hos d-His, d-Phe, l-DOPA, l-Trp, serotonin , thiab qee cov pyridyl-alkylamines tau zoo dua activators, nrog K(A) nyob rau hauv thaj tsam ntawm 13-19 microM.Qhov zoo tshaj plaws CA VI activators yog l-Phe, d-DOPA, l-Tyr, 4-amino-l-Phe, thiab histamine, nrog K(A)s nyob rau hauv thaj tsam ntawm 1.23-9.31 microM.Tag nrho cov activators txhim kho k (miv), tsis muaj kev cuam tshuam rau K (M), koom nrog yog li hauv kev txiav txim siab cov kauj ruam hauv lub voj voog catalytic, cov kev hloov pauv proton ntawm cov enzyme nquag thiab ib puag ncig.