Cov khoom

Siv los tsim CAPS tsis, zwitterionic tsis muaj txiaj ntsig hauv thaj tsam ntawm pH 7.9-11.1.CAPS tsis yog siv dav hauv Western thiab immunoblotting thwmsim nrog rau protein sequencing thiab txheeb xyuas.Siv nyob rau hauv lub electro hloov ntawm cov proteins rau PVDF (sc-3723) los yog nitrocellulose daim nyias nyias (sc-3718, sc-3724).Qhov pH siab ntawm qhov tsis zoo no ua rau nws muaj txiaj ntsig zoo rau kev hloov pauv cov protein nrog pI> 8.5.thiab tsawg reactivity nrog enzymes los yog proteins, tsawg ntsev los.

Hauv cheeb tsam capillary electrophoresis lub electrophoretic tshaj tawm ntawm ib qho ion txo qis vim qhov concentration ntawm cov tshuaj electrolyte tom qab yog nce.Qhov no yog tshwm sim los ntawm kev hloov pauv ntawm electrophoretic txav ntawm ion (muep) nrog rau kev hloov pauv ntawm lub zog net cuam tshuam rau nws, uas yog lub zog hluav taws xob zoo (Eeff).Lub electrophoretic mobility ntawm ib ion yog hloov los ntawm kev hloov nyob rau hauv kiag li viscosity ntawm cov tshuaj electrolyte thiab hloov nyob rau hauv lub solvated loj ntawm ion.Eeff tau hloov pauv tsuas yog los ntawm kev hloov pauv ntawm qhov loj ntawm cov txiaj ntsig asymmetry thiab cov nyhuv electrophoretic, ob qho tib si ntawm cov retard cov lus tsa suab ntawm ions.Hauv txoj kev tshawb no, cov txheej txheem peb-kos npe tau siv los kawm txog cov txiaj ntsig ntawm keeb kwm ntawm electrolyte concentration (0.02-0.08M 3-[cyclohexylamino]-1-propanesulfonic acid thiab counter ion (Li, Na, K, thiab Cs) ntawm Eeff. Nws tau pom tias qhov concentration ntawm cov electrolyte tom qab muaj feem cuam tshuam rau Eeff thiab Eeff mus txog E raws li qhov concentration ntawm cov electrolyte tom qab zero zero. Lub txee ion muaj qhov cuam tshuam me me ntawm Eeff: raws li qhov loj ntawm cov hydrated radius ntawm lub txee ion nce. , Eeff txo qis. Cov txheej txheem peb-kos npe tau ua pov thawj tias muaj txiaj ntsig zoo rau kev txiav txim siab zoo li no.