Cov khoom

Kev tsom xam ntawm cov txheej xwm proteome-wide phosphorylation tseem yog ib qho kev sib tw tseem ceeb vim yog qhov nyuaj ntawm cov protein phosphorylation ntau heev.Hauv kev ua haujlwm no, peb ntsuas qhov ua tiav ntawm Lys-N, Lys-C, thiab trypsin hais txog lawv lub peev xwm los pab txhawb kev tshawb fawb thoob ntiaj teb ntawm phosphoproteome.Ib qho kev ua kom zoo ntawm cov pH muaj zog cation txauv tau siv los ua kom muaj txiaj ntsig zoo cais N-terminally acetylated, phosphorylated, thiab nonmodified peptides.Tag nrho ntawm 5036 nonredundant phosphopeptides tuaj yeem txheeb xyuas nrog qhov kev tshawb pom tsis tseeb ntawm <1% los ntawm 1 mg ntawm cov protein siv kev sib xyaw ntawm peb enzymes.Peb cov ntaub ntawv tau qhia tias qhov sib tshooj ntawm cov ntaub ntawv phosphopeptide tsim nrog cov proteases sib txawv yog marginal, whereas qhov sib tshooj ntawm ob qho tib si tsim cov ntaub ntawv tryptic tau pom tias muaj tsawg kawg yog 4 zaug siab dua.Nyob rau hauv txoj kev no, qhov sib npaug ntawm kev siv Lys-N thiab trypsin tau ua kom muaj 72% nce ntawm tus lej ntawm cov tshuaj phosphopeptides piv rau d rau trypsin ib leeg, thaum trypsin replicate kev sim tsuas yog ua rau nce 25%.Yog li, thaum tsom mus rau cov ntaub ntawv trypsin thiab Lys-N nkaus xwb, peb tau txheeb xyuas 4671 nonredundant phosphopeptides.Kev tshuaj xyuas ntxiv ntawm cov chaw kuaj pom tau pom tias Lys-N thiab trypsin cov ntaub ntawv teev tau ua kom muaj txiaj ntsig zoo hauv cov qauv phosphorylation sib txawv, ntxiv pov thawj tias cov txheej txheem multiprotease muaj txiaj ntsig zoo hauv kev tshuaj ntsuam phosphoproteome.