Guanidine Hydrochloride Cas: 50-01-1 99% Dawb hmoov
| Catalog Number | xwm 90198 |
| Khoom npe | Guanidine Hydrochloride |
| CAS | 50-01-1 |
| Molecular Formula | CH6N3 Cl |
| Molecular Luj | 95.5388 ib |
| Cov ntaub ntawv khaws cia | Ambient |
| Harmonized tariff Code | 29252900 ib |
Khoom Specification
| Melting Point | 185.3 - 187.5 Deg C |
| Dej | <0.3% |
| Solubility | Clear colorless tov ntawm 6M hauv Dej |
| Assay | > 99% |
| UV Absorbance | 0.10 ntawm 260nm |
| Qhov tshwm sim | Dawb hmoov |
| IR spectrum | Ua raws |
Ib qho ntawm cov xwm txheej tiv thaiv kev siv tshuaj kho mob rau cov kab mob pob txha degenerative yog qhov tsim nyog ntawm cov hlwb.Chondrocytes, tsuas yog hom pob txha mos, loj hlob hauv vitro nyob rau hauv kab lis kev cai kom nthuav cov xov tooj ntawm tes poob lawv cov phenotype nrog rau lub peev xwm los tsim cov nqaij mos hyaline cartilaginous.Nyob rau hauv txoj kev tshawb no peb txiav txim siab hais tias ib tug serum- thiab loj hlob-yam-dawb peb-dimensional (3D) kab lis kev cai system restores lub peev xwm ntawm cov passaged chondrocytes los tsim cov pob txha mos nyob rau hauv vitro, ib tug txheej txheem uas muaj xws li sox9.Bovine articular chondrocytes tau kis ob zaug rau. tso cai rau cov xov tooj ntawm tes nthuav dav (P2) thiab kab lis kev cai ntawm qhov ntom ntom ntawm 3D collagen-type-II-coated membranes hauv cov ntsiab lus qabzib siab ntxiv nrog insulin thiab dexamethasone (SF3D).Cov hlwb tau tshwm sim tom qab monolayer nthuav dav thiab ua raws li 3D kab lis kev cai los ntawm kev ntws cytometry, noob qhia, thiab histology.Cov kev hloov pauv thaum ntxov hauv kev taw qhia kev hloov pauv thaum lub sij hawm redifferentiat ion yog tus yam ntxwv.Lub P2 hlwb tau pom ib tug progenitor-zoo li antigen profile ntawm 99% CD44(+) thiab 40% CD105(+) thiab ib tug gene qhia profile qhia ntawm interzone hlwb.P2 nyob rau hauv SF3D qhia chondrogenic noob thiab khaws cov extracellular matrix.Downregulating insulin receptor (IR) nrog HNMPA-(AM3) lossis PI-3 / AKT kinase txoj hauv kev (ua haujlwm los ntawm kev kho tshuaj insulin) nrog Wortmannin inhibited collagen synthesis.HNMPA-(AM3) txo qis kev qhia ntawm Col2, Col11, thiab IR noob ntxiv rau Sox6 thiab -9.Co-immunoprecipitation thiab chromatin immunoprecipitation tsom xam ntawm HNMPA-(AM3)-kho hlwb pom kev khi ntawm cov coactivators Sox6 thiab Med12 nrog Sox9 tab sis txo Sox9-Col2a1 binding.Peb piav qhia txog cov kab lis kev cai tshiab uas tso cai rau kev nce hauv cov chondrocytes thiab txhawb nqa. Hyaline-zoo li pob txha mos tsim nyob rau hauv ib feem los ntawm insulin-mediated Sox9-Col2a1 khi.Qhov tsim nyog ntawm cov ntaub so ntswg tsim los ntawm txoj hauv kev no rau kev siv hauv kev sib koom ua ke yuav tsum tau kuaj xyuas hauv vivo.
