Cov khoom

Mycobacterium tuberculosis tuaj yeem nyob rau ntau xyoo hauv tib neeg tus tswv tsev.Cov lus teb nruj me ntsis cuam tshuam nrog inorganic polyphosphate [poly(P)], uas yog synthesized thiab hydrolyzed los ntawm polyphosphate kinase (PPK) thiab exopolyphosphatase (PPX), feem, ntseeg tau ua lub luag haujlwm tseem ceeb hauv cov kab mob persistence.Peb qhia ntawm no tias M. tuberculosis poly(P) tsub zuj zuj yog ib ntus txuas rau kev txwv kev loj hlob ntawm bacillary.Peb kuj txheeb xyuas M. tuberculosis Rv1026 ua ib qho tshiab exopolyphosphatase nrog hydrolytic kev ua si tiv thaiv ntev-chain poly(P).Siv cov txheej txheem tetracycline-inducible qhia kom txo qis kev qhia ntawm Rv1026 (ppx2), peb pom tias M. tuberculosis poly(P) tsub zuj zuj ua rau kev loj hlob qeeb thiab txo qis rau isoniazid, muaj zog tiv thaiv tshav kub thiab kua qaub pH, thiab txhim kho intracellular ciaj sia taus. thaum lub sij hawm tus kab mob macrophage.Los ntawm kev sib kis electron microscopy, lub ppx2 knockdown strain tau nthuav tawm cov phab ntsa ntawm phab ntsa tuab, uas tau cuam tshuam nrog rov tsim cov phab ntsa ntawm phab ntsa permeability rau cov tshuaj hydrophilic es tsis yog induction ntawm cov tshuaj efflux twj los yog hloov biofilm tsim txheeb ze rau qhov khoob vector tswj.Kev txheeb xyuas cov ntaub ntawv thiab cov txheej txheem metabolomic tau nthuav tawm qhov kev hloov pauv ntawm cov metabolism hauv ppx2 knockdown yam ntxwv los ntawm kev txo qis thiab kev txhais lus thiab kev poob qis ntawm qib glycerol-3-phosphate.Hauv cov ntsiab lus, poly(P) ua lub luag haujlwm tseem ceeb hauv M. tuberculosis kev loj hlob txwv thiab metabolic downshift thiab pab txhawb rau cov tshuaj tiv thaiv kab mob los ntawm kev hloov pauv ntawm phab ntsa ntawm phab ntsa permeability.Cov lus teb nruj, nrog rau kev tswj cov molecules inorganic polyphosphate [poly(P)] thiab (p ) ppGpp, ntseeg tau los kho Mycobacterium tuberculosis persistence.Hauv txoj kev tshawb no, peb tau txheeb xyuas cov enzyme tshiab (Rv1026, PPX2) lub luag haujlwm rau hydrolyzing long-chain poly(P).Ib qho kev tsim kho caj ces M. tuberculosis strain deficiency nyob rau hauv ppx2 gene pom tau tias ntau dua poly(P) ntau ntau, uas tau txuam nrog cov kab mob loj hlob sai thiab txo susce ptibility rau thawj kab tshuaj isoniazid, nrog rau cov kab mob muaj sia nyob ntxiv thaum raug kev nyuaj siab. cov xwm txheej thiab hauv macrophages.Tus txheeb ze ntawm kev tswj hwm, cov mutant pom cov thickness ntawm phab ntsa ntawm tes thiab txo cov tshuaj permeability.Kev nthuav qhia cov noob caj noob ces thoob ntiaj teb thiab kev tshuaj xyuas metabolite tau qhia txog kev txo qis ntawm cov tshuab hloov pauv thiab txhais lus thiab hloov pauv hauv kev siv cov pa roj carbon.Hauv cov ntsiab lus, kev tswj hwm ntawm poly(P) tshuav nyiaj li cas yog qhov tseem ceeb rau kev tsim cov kab mob M. tuberculosis.