GSH Cas: 70-18-8 98% Dawb crystalline hmoov
| Catalog Number | xwm 90227 |
| Khoom npe | GSH |
| CAS | 70-18-8 ib |
| Molecular Formula | C10H17N3O6S |
| Molecular Luj | 307.323 ib |
| Cov ntaub ntawv khaws cia | 2 txog 8 ° C |
| Harmonized tariff Code | 29 3098 ib |
Khoom Specification
| Khoom Specification | |
| Melting Point | 185 ° C |
| Qib | Pharma Qib |
| Cov hlau hnyav | 10 ppm max |
| Arsenic | 2 ppm max |
| Kev txheeb xyuas | IR : Zoo ib yam nrog Cov Kev Siv Spectrum |
| Poob rau ziab | 0.5% max |
| Cia Kub | +20 ° C |
| Molecular Luj | 612.63 ib |
| Assay | 98.0% min |
| Kev sib hloov kho qhov muag | -15.5 ° - 17.5 ° C |
| Residue ntawm Ignition | 0.1% max |
| Clarity of Solution | Ntshiab thiab tsis muaj xim |
| Qhov tshwm sim | Dawb crystalline hmoov |
| Yam khoom | Tag Nrho: 2.0% max, GSSG: 1.5% max |
ORF45 protein ntawm Kaposi's sarcoma-associated herpesvirus (KSHV) yog gammaherpesvirus-tshwj xeeb tam sim-thaum ntxov tegument protein.Peb cov kev tshawb fawb yav dhau los tau qhia txog nws lub luag haujlwm tseem ceeb hauv ob qho tib si thaum ntxov thiab lig ntawm KSHV kab mob.Hauv txoj kev tshawb no, peb tau tshawb xyuas qhov sib cuam tshuam ntawm ORF45 siv lub vaj huam sib luag ntawm cov tshuaj tiv thaiv monoclonal.Ntxiv rau qhov yav tas los txheeb xyuas cov txheej txheem tswj hwm kinase (ERK) thiab p90 ribosomal S6 kinase (RSK) cov proteins, peb pom ob peb lwm copurified proteins, suav nrog cov tseem ceeb ntawm ∼38 kDa thiab ∼130 kDa.Mass spectrometry qhia tias 38-kDa protein yog kab mob ORF33 thiab 130-kDa protein yog cellular USP7 (ubiquitin-specific protease 7).Peb tau kos npe rau ORF33-binding domain rau qhov kev txuag carboxyl-terminal 19 amino acids (aa) ntawm ORF45 thiab USP7-binding domain rau cov lus pom zoo qhia hauv cheeb tsam nruab nrab ntawm ORF45.Siv immunofluorescence staining, peb pom colocalization ntawm ORF45 nrog ORF33 lossis USP7 ob qho tib si nyob rau hauv cov kab mob sib kis thiab hauv cov kab mob KSHV.Ntxiv mus, peb pom ORF45-dependent relocalization ntawm ib feem ntawm ORF33/USP7 los ntawm lub nucleus mus rau cytoplasm.Peb pom tias ORF45 ua rau muaj kev nce hauv ORF33 protein ntau ntxiv uas raug tshem tawm yog tias ORF33- lossis USP7-binding domain hauv ORF45 raug tshem tawm.Tsis tas li ntawd, kev tshem tawm ntawm kev khaws cia carboxyl terminus ntawm ORF45 hauv KSHV genome tau txo qis ORF33 protein nyob rau hauv KSHV-cov kab mob hlwb thiab tshem tawm kev tsim cov noob virions.Sib sau ua ke, peb cov txiaj ntsig tsis tsuas yog nthuav tawm cov khoom tshiab ntawm ORF45 interactome, tab sis kuj qhia tau tias kev sib cuam tshuam ntawm cov proteins no yog qhov tseem ceeb rau KSHV lytic replication.Kaposi's sarcoma-associated herpesvirus (KSHV) yog tus neeg sawv cev ntawm ntau tus neeg mob qog noj ntshav.KSHV ORF45 yog cov protein ntau uas yuav tsum tau ua rau KSHV lytic replication, tab sis cov txheej txheem tseeb uas ORF45 ua nws cov haujlwm tseem ceeb tsis meej.Peb cov kev tshawb fawb yav dhau los tau qhia tias tag nrho ORF45 pr otein hauv hlwb muaj nyob rau hauv high-molecular-yuag complexes.Yog li ntawd peb tau nrhiav kev ua tus cwj pwm ntawm kev sib cuam tshuam ntawm ORF45 los muab kev nkag siab rau nws lub luag haujlwm thaum lub sij hawm lytic replication.Siv lub vaj huam sib luag ntawm cov tshuaj tiv thaiv kab mob monoclonal, peb tau soj ntsuam ORF45 interactome hauv KSHV-cov kab mob.Peb tau txheeb xyuas ob tus neeg koom tes tshiab ntawm ORF45: cov kab mob kis kab mob ORF33 thiab cellular ubiquitin-specific protease 7 (USP7).Peb qhia ntxiv tias kev sib cuam tshuam ntawm ORF45 thiab ORF33 yog qhov tseem ceeb rau kev tsim cov KSHV cov kab mob kis tau zoo, qhia tias qhov kev cuam tshuam cuam tshuam nrog qhov kev cuam tshuam no tuaj yeem sawv cev rau lub tswv yim tshiab los tiv thaiv KSHV lytic replication.
