Cov khoom

Cov txheej txheem staining yog piav qhia rau kev siv nrog glycol methacrylate embedded cov ntaub so ntswg uas tsis stain lub yas embedment los yog tshem tawm cov seem ntawm cov iav slides.Cov xim yooj yim yog celestine xiav B. Nws yog npaj los ntawm kev kho 1 g ntawm cov xim nrog 0.5 ml concentrated sulfuric acid.Nws yog tom qab ntawd yaj nrog cov tshuaj hauv qab no.Ntxiv 14 ml glycerine rau 100 ml 2.5% ferric ammonium sulfate thiab sov cov tshuaj rau 50 C. Thaum kawg kho cov pH rau 0.8 mus rau 0.9 Cov kua qaub staining muaj 0.075% ponceau de xylidine thiab 0.025% acid fuchsin hauv 10% acetic acid.Cov zawv zawg uas muaj cov yas qhuav qhuav tau muab tso rau hauv cov tshuaj celestine xiav rau tsib feeb thiab hauv cov tshuaj ponceau-fuchsin rau kaum feeb nrog cov dej cuam tshuam.Tom qab ntxuav tas, cov seem yog cua qhuav thiab npog.Cov txheej txheem staining no ua rau cov ntaub so ntswg zoo ib yam li cov txheej txheem hematoxylin thiab eosin.