Ib qho tshuaj tiv thaiv monoclonal (mAb 8281) tshwj xeeb rau lub davhlau ya nyob twg β-galactose (βGal) ntawm glycosphingolipids (GSL) thiab glycoproteins tau tsim los ntawm cov nas txhaj tshuaj nrog lipid extract los ntawm cov kab mob lymphocytic leukemia (ALL) tshiab.Immuno-thin txheej chromatography (ITLC) thiab kev soj ntsuam kev sib tw nrog cov qauv purified nruab nrab GSL, cov suab thaj dawb, thiab hluavtaws neoglycoproteins pom mAb 8281 ua kom muaj zog nrog LacCer, GalCer thiab Gal-β-O-(CH3)2S(CH3)2- CONH-(Gal-β-O-CETE) txuas rau bovine serum albumin (BSA).Cov qab zib penultimate kuj ua lub luag haujlwm hauv kev khi.Cov tshuaj tiv thaiv kab mob tsis ua haujlwm nrog cov carbohydrates nrog lub davhlau ya nyob twg αGal cov qauv thiab tsis cuam tshuam nrog lub davhlau ya nyob twg.Indirect immunoperoxidase staining thiab ntws cytometry nrog mAb 8281 pom zoo staining ntawm ntau cov ntaub so ntswg, nrog rau cov nqaij du, plab hnyuv mucosa, lymph node B hlwb thiab monocytes.ITLC tsom xam ntawm GSL muaj pes tsawg leeg ntawm B cell neoplasms tshiab siv mAb 8281 tau lees paub tias muaj lactosylceramide thiab galactosylceramide hauv neoplasms ntawm ntau theem ntawm kev sib txawv.Vim tias nws qhov tshwj xeeb rau lub davhlau ya nyob twg βGal carbohydrate residues, mAb 8281 tej zaum yuav muaj txiaj ntsig zoo hauv cov qauv thiab kev ua haujlwm ntawm GSL.
