4-Nitrophenyl phosphate disodium ntsev 6-hydrate CAS: 333338-18-4 dawb rau daj daj hmoov
| Catalog Number | xwm 90511 |
| Khoom npe | 4-Nitrophenyl phosphate disodium ntsev 6-hydrate |
| CAS | 333338-18-4 |
| Molecular Formula | C6H16Na2O12P |
| Molecular Luj | 371.142 ib |
| Cov ntaub ntawv khaws cia | Ambient |
| Harmonized tariff Code | 29190 000 Nws |
Khoom Specification
| Qhov tshwm sim | dawb mus daj daj |
| Assay | 99% |
Tshaj li 70% ntawm cov qib qis gliomas muaj kev hloov pauv ntawm heterozygous R132H hauv cov noob coding rau isocitrate dehydrogenase 1 (IDH1).Qhov no muab cov enzyme nrog lub peev xwm tshiab los hloov α-ketoglutarate rau 2-hydroxyglutarate, thaum kawg ua rau qog nqaij hlav.Lub hauv paus loj ntawm 2-hydroxyglutarate ntau lawm yog glutamine, uas, hauv kev mob qog noj ntshav, kuj yog qhov chaw rau tricarboxylic acid cycle (TCA) anaplerosis.Lwm qhov chaw ntawm anaplerosis yog pyruvate flux ntawm pyruvate carboxylase (PC), uas yog ib txoj hauv kev zoo hauv astrocytes.Lub hom phiaj ntawm txoj kev tshawb no yog los txiav txim seb PC puas ua haujlwm los ntawm TCA anaplerosis hauv IDH1 mutant hlwb nyob rau hauv glutamine yog siv rau 2-hydroxyglutarate ntau lawm.Immortalized tib neeg astrocytes engineered los qhia heterozygous mutant IDH1 los yog qus-hom IDH1 tau tshawb xyuas.Flux ntawm pyruvate ntawm PC thiab ntawm pyruvate dehydrogenase (PDH) tau txiav txim los ntawm kev siv magnetic resonance spectroscopy los soj ntsuam cov ntawv lo ntawm [2-¹³C]glucose-derived ¹³C-labe coj glutamate thiab glutamine.Kev soj ntsuam kev ua si, RT-PCR thiab western blotting tau siv los soj ntsuam qhov qhia thiab kev ua ntawm cov enzymes cuam tshuam.Cov ntaub ntawv Cancer Genome Atlas (TCGA) tau txheeb xyuas los ntsuas qhov kev qhia ntawm cov enzymes hauv tib neeg glioma cov qauv.Piv rau cov hom tsiaj qus, mutant IDH1 hlwb ua rau muaj feem cuam tshuam ntawm PC.Qhov no tau cuam tshuam nrog kev nce ntxiv hauv PC kev ua si thiab kev qhia.Raws li qhov tshwm sim, PDH kev ua haujlwm tau txo qis, yuav ua rau muaj kev sib haum xeeb los ntawm inhibitory PDH phosphorylation los ntawm PDH kinase 3. Raws li kev soj ntsuam hauv hlwb, kev soj ntsuam ntawm TCGA cov ntaub ntawv qhia tau hais tias muaj kev nce ntxiv hauv PC qhia hauv mutant IDH-expressing human glioma kuaj piv rau cov tsiaj qus- Hom IDH.Peb qhov kev tshawb pom qhia tias kev hloov pauv hauv PC thiab PDH tej zaum yuav yog ib feem tseem ceeb ntawm kev hloov ntawm cov cellular hloov mus rau IDH1 kev hloov pauv thiab tuaj yeem ua lub hom phiaj kho mob.
