Ib qho tshiab β-glucosidase gene, bgl1G5, tau cloned los ntawm Phialophora sp.G5 thiab tau ua tiav hauv Pichia pastoris.Kev soj ntsuam ib ntus qhia tias cov noob muaj 1,431-bp qhib kev nyeem ntawv encoding cov protein ntawm 476 amino acids.Lub deduced amino acid sequence ntawm bgl1G5 qhia ib tug siab tus kheej ntawm 85% nrog ib tug characterized β-glucosidase los ntawm Humicola grisea ntawm glycoside hydrolase tsev neeg 1. Piv nrog rau lwm yam fungal counterparts, Bgl1G5 qhia zoo sib xws kev pom zoo ntawm pH 6.0 thiab 50 ° C thiab ruaj khov. ntawm pH 5.0-9.0.Ntxiv mus, Bgl1G5 nthuav tawm qhov ntsuas kub zoo ntawm 50 ° C (6 h ib nrab-lub neej) thiab kev ua haujlwm siab dua (54.9 U mg-1).K m thiab V max qhov tseem ceeb ntawm p-nitrophenyl β-D-glucopyranoside (pNPG) yog 0.33 mM thiab 103.1 μmol min-1 mg-1, raws li.Qhov kev ntsuam xyuas tshwj xeeb hauv substrate tau pom tias Bgl1G5 muaj zog tiv thaiv pNPG, tsis muaj zog ntawm p-nitrophenyl β-D-cellobioside (pNPC) thiab p-nitrophenyl-β-D-galactopyranoside (ONPG), thiab tsis muaj kev ua haujlwm ntawm cellobiose.
