Ib tug beta-glucosidase noob (bgl3) los ntawm Streptomyces sp.QM-B814 (American Type Culture Collection 11238) tau raug cloned los ntawm kev ua haujlwm ntxiv ntawm beta-glucosidase-tsis zoo mutant ntawm Streptomyces lividans.Ib qho qhib kev nyeem ntawv ntawm 1440 nucleotides encoding polypeptide ntawm 479 amino acids tau pom los ntawm kev sib txuas.Lub encoded protein (Bgl3) qhia tau hais tias nws kim heev (tshaj li 45% tus kheej) nrog beta-glycosidases los ntawm tsev neeg-1 glycosyl hydrolases.Lub cloned enzyme, purified tom qab ammonium sulphate nag lossis daus thiab ob kauj ruam chromatographic, yog monomeric nrog molecular mass 52.6 kDa, raws li txiav txim los ntawm huab hwm coj spectrometry, thiab ib qho isoelectric point ntawm pI 4.4.Cov enzyme zoo li yog beta-glucosidase nrog cov txheej txheem dav dav, ua haujlwm ntawm cellooligomers, thiab ua cov tshuaj tiv thaiv transglycosylation.Qhov kwv yees pom tseeb Km qhov tseem ceeb rau p-nitrophenyl-beta-D-glucopyranoside thiab cellobiose yog 0.27 mM thiab 7.9 mM, feem.Cov txiaj ntsig Ki rau cov piam thaj thiab delta-gluconolactone, siv p-nitrophenyl-beta-D-glucopyranoside ua substrate, yog 65 mM thiab 0.08 mM, raws li.Lub purified enzyme muaj pH zoo ntawm pH 6.5 thiab qhov kub zoo rau kev ua haujlwm yog 50 degrees.
