Cov khoom

4-Methylumbelliferyl β-D-glucopyranoside tau siv los ua substrate: · hauv glucosylceramidase β enzyme kev soj ntsuam hauv lysosome-enriched fractions los ntawm thawj hippocampal neurons · hauv β-glucosidase assay thaum lub sij hawm poov xab fermentation · rau kev soj ntsuam glucose-1GBA (glucosidase) Kev ua haujlwm hauv macrophage cell line (RAW)

4-Methylumbelliferyl β-D-glucopyranoside yog ib qho hluavtaws enzymatic substrate rau glycosidase.Nws tau siv los ua substrate rau β-glucosidase los ntawm enterococci.

Peb tshaj tawm tias cov neeg pub dawb glycoside yooj yim tuaj yeem hloov pauv qhov sib npaug ntawm glycosyltransferase-catalyzed cov tshuaj tiv thaiv, hloov NDP-qab zib tsim los ntawm endothermic mus rau txheej txheem exothermic.Txhawm rau ua kom pom kev siv hluav taws xob ntawm qhov kev hloov pauv ntawm qhov ntsuas kub no, peb qhia txog glycosyltransferase-catalyzed synthesis ntawm 22 qab zib nucleotides los ntawm cov neeg muaj suab thaj yooj yim, nrog rau qhov sib txuam hauv qhov tsim cov piam thaj nucleotides raws li kev tsav tsheb hauv cov ntsiab lus ntawm glycosyltransferase-catalyzed cov tshuaj tiv thaiv. me-molecule glycodiversification.Cov neeg muaj ntxhiab tsw yooj yim no kuj tau ua kom muaj kev ntsuam xyuas dav dav rau glycosyltransfer, muaj feem xyuam rau kev tshawb nrhiav tshuaj, protein engineering thiab lwm yam kev tshawb nrhiav cov piam thaj hauv nucleotide.Txoj kev tshawb no ncaj qha cuam tshuam qhov kev xav dav dav uas NDP-qab zib yog 'siab-zog' cov neeg mob ntshav qab zib thaum muab tawm ntawm lawv cov ntsiab lus lom neeg ib txwm muaj.