Cov khoom

Txoj kev tshawb no tau ua los tshawb xyuas cov txiaj ntsig ntawm cov zaub soy peptides ntawm cov neeg laus qia hlwb (ASCs) thaum tsis muaj ntshav qab zib thiab lawv cov txheej txheem ua tau.Kev loj hlob ntawm tib neeg adipose cov ntaub so ntswg-derived mesenchymal stem cells (ADSCs) thiab cov hlab ntshav-derived mesenchymal qia hlwb (CB-MSCs) kho nrog soy peptides tau pom tias nce ntxiv rau 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide thiab Click-iT 5-ethynyl-2'-deoxyuridine flow cytometry assay.Tsis tas li ntawd, cov kua peptides tau coj mus rau cov txheej txheem phosphorylation ntawm p44/42 MAPK (ERK), mammalian lub hom phiaj ntawm rapamycin (mTOR), p70 S6 kinase, S6 ribosomal protein (S6RP) thiab eukaryotic initiation factor 4E (eIF4E) hauv ADSCs.Tsis tas li ntawd, kev soj ntsuam ntau ntawm cov cytokines tau qhia tias kev tsim cov hloov pauv kev loj hlob zoo-beta1 (TGF-β1), vascular endothelial kev loj hlob zoo thiab interleukin-6 tau nce ntau hauv cov lus teb rau kev kho mob soypeptides hauv ob qho tib si ADSCs thiab CB-MSCs.Ib yam li ntawd, soy peptide-induced phosphorylation ntawm ERK / mTOR / S6RP / eIF4E txoj hauv kev tau raug thaiv nyob rau hauv cov lus teb rau pretreatment nrog PD98059, ib qho tshwj xeeb ERK inhibitor.Ntxiv mus, inhibition ntawm TGF-β1 los ntawm PD98059 pretreatment thiab qhov sib law liag ntawm ADSC proliferation qhia tias TGF-β1 induces phosphorylation ntawm mTOR/S6RP/eIF4E.Sib sau ua ke, cov txiaj ntsig ntawm txoj kev tshawb no qhia tias ERK-dependent zus tau tej cov TGF-β1 plays lub luag hauj lwm tseem ceeb nyob rau hauv cov kua peptide-induced proliferation ntawm ADSCs nyob rau hauv serum-dawb tej yam kev mob.Copyright © 2012 Elsevier Inc. All rights reserved.