Cov khoom

Tricine, yog zwitterionic tsis reagent uas nws lub npe tau muab los ntawm Tris thiab glycine.Nws cov qauv zoo ib yam li Tris, tab sis nws cov concentration siab muaj zog inhibitory kev ua si dua Tris.Ib qho ntawm Good's buffer reagents, Ameslikas tsim los muab lub kaw lus tsis rau cov tshuaj tiv thaiv chloroplast.Qhov zoo pH tsis zoo ntawm Tricine yog 7.4-8.8, pKa = 8.1 (25 ° C), thiab nws feem ntau yog siv los ua kev khiav tsis zoo thiab rau kev rov ua cov cell pellets.Tricine muaj cov yam ntxwv ntawm cov nqi tsis zoo thiab lub zog ionic siab, uas yog qhov tsim nyog rau kev sib cais electrophoretic ntawm cov protein uas tsis muaj molecular hnyav ntawm 1 ~ 100 kDa.Nyob rau hauv qhov kev ntsuam xyuas ntawm firefly luciferase-based ATP, piv rau 10 qhov tsis sib xws, Tricine (25 mM) tau pom cov txiaj ntsig zoo tshaj plaws.Tsis tas li ntawd, Tricine kuj yog ib qho zoo hydroxyl radical scavenger hauv cov dawb radical-induced membrane puas thwmsim.