Cov khoom

Hauv cov tsiaj feem ntau uas muaj X thiab Y chromosomes, chromosome-wide mechanisms yog siv los sib npaug X-txuas gene qhia hauv txiv neej thiab poj niam.Nyob rau hauv yoov Drosophila melanogaster, ntau npaum li cas kev them nyiaj mechanism kuj feem ntau txuas mus rau X-txuas transgenes.Ntau tshaj 70 cov kab transgenic ntawm Australian yaj blowfly Lucilia cuprina tau ua ib feem ntawm kev siv zog los tsim cov txiv neej-tsuas yog hom kab mob rau kev tswj caj ces ntawm cov kab no loj ntawm yaj.Tag nrho cov kab muaj ib tug constitutively qhia fluorescent protein marker noob.Nyob rau hauv tag nrho 12 X-txuas kab, poj niam larvae qhia brighter fluorescence tshaj li txiv neej larvae, tawm tswv yim cov cim noob caj noob ces yog tsis npaum li cas compensated.Qhov no tau lees paub los ntawm ntau qhov RT-PCR rau cov kab xaiv.Txhawm rau txiav txim siab seb cov noob caj noob ces endogenous X-txuas ntxiv tau them nyiaj ntau npaum li cas, peb cais 8 cov noob uas yog orthologs ntawm cov noob uas nyob ntawm plaub chromosome hauv D. melanogaster.Cov pov thawj tsis ntev los no qhia tias D. melanogaster plaub chromosome, los yog Muller element F, yog poj koob yawm txwv X chromosome hauv Diptera uas tau rov qab mus rau ib qho autosome hauv Drosophila hom.Peb qhia los ntawm ntau PCR ntawm txiv neej thiab poj niam DNA uas 6 ntawm 8 pawg F txuas txuas nyob ntawm X chromosome hauv L. cuprina.Lwm ob lub Muller element F noob tau pom tias yog autosomal hauv L. cuprina, thaum ob lub Muller element B noob tau pom nyob rau tib cheeb tsam ntawm X chromosome li L. cuprina orthologs ntawm D. melanogaster Ephrin thiab gawky noob.Peb pom tias L. cuprina X chromosome genes yog sib npaug ntawm cov txiv neej thiab poj niam (piv txwv li, tag nrho cov nyiaj them rov qab).Yog li, tsis zoo li hauv Drosophila, nws zoo nkaus li tias Lucilia qhov kev them nyiaj ntau npaum li cas yog qhov tshwj xeeb rau cov noob endogenous rau X chromosome thiab tsis tuaj yeem sib koom ua ke los ntawm cov transgenes uas nyuam qhuav tuaj txog.