Cov khoom

Protein phosphorylation tswj ntau lub zog ntawm tes thiab ua lub luag haujlwm tseem ceeb hauv kev loj hlob ntawm cov nroj tsuag.Txhawm rau ntxiv thiab nthuav dav rau yav dhau los kev tshawb fawb ntawm cov protein phosphorylation hauv Arabidopsis cov noob peb siv lwm txoj hauv kev uas muab cov protein rho tawm hauv cov xwm txheej uas tsis yog denaturing nrog immobilized hlau-ion affinity chromatography (IMAC) enrichment ntawm cov phosphoproteins tsis tu ncua hauv Rubisco-depleted extracts, ua raws li kev txheeb xyuas. siv ob-dimensional gel electrophoresis (2-DE) thiab kua chromatography-tandem mass spectrometry (LC-MS/MS).Hauv-gel trypsin zom thiab tshuaj xyuas ntawm cov chaw xaiv gel tau txheeb xyuas 144 phosphorylated peptides thiab residues, ntawm uas tsuas yog 18 phosphopeptides thiab 8 phosphosites tau pom nyob rau hauv PhosPhAt 4.0 thiab P3DB Arabidopsis thaliana phosphorylation sites.Ntau tshaj li ib nrab ntawm 82 txheeb xyuas phosphoproteins tau koom nrog hauv carbohydrate metabolism, photosynthesis / ua pa lossis oxidative kev nyuaj siab teb mechani sms.Txhim kho cov phosphoproteins tsis zoo ua ntej 2-DE thiab LC-MS / MS zoo li txhim kho kev tshawb pom ntawm phosphorylated threonine thiab tyrosine residues piv nrog cov txheej txheem uas siv peptide-theem enrichment, qhia tias ob txoj hauv kev yog me ntsis ntxiv nyob rau hauv cov nqe lus ntawm phosphorylation site coverage.Sib piv cov txiaj ntsig rau cov tub ntxhais hluas cov tub ntxhais hluas uas tau txais yav dhau los rau cov laus Arabidopsis nplooj tau txheeb xyuas tsib cov proteins uas sib txawv phosphorylated hauv cov ntaub so ntswg, ua kom pom qhov muaj peev xwm ntawm cov txheej txheem no rau kev tshawb xyuas qhov muaj zog ntawm cov protein phosphorylation thaum lub sij hawm cog qoob loo.