Phosphatase, alkaline CAS: 9001-78-9 dawb, grey dawb los yog txiv duaj monoclinic siv lead ua los yog hmoov
| Catalog Number | xwm 90379 |
| Khoom npe | Phosphatase, alkaline |
| CAS | 9001-78-9 |
| Molecular Formula | C21H36N8O6 |
| Molecular Luj | 496.57 ib |
| Cov ntaub ntawv khaws cia | 2 txog 8 ° C |
| Harmonized tariff Code | 3 50790 |
Khoom Specification
| Assay | 99% |
| Qhov tshwm sim | dawb, grey dawb los yog txiv duaj monoclinic siv lead ua los yog hmoov |
Txawm hais tias cov tshuaj fluoride paub los txhawb kev tsim cov pob txha, cov txheej txheem hauv qab no tsis to taub tag nrho.Cov kev tshawb fawb tsis ntev los no tau cuam tshuam txog Wnt / β-catenin txoj hauv kev ua ib qho kev qhia tseem ceeb hauv cov pob txha biology.Peb cov kev tshawb fawb yav dhau los tau qhia txog lub luag haujlwm tseem ceeb ntawm txoj hauv kev canonical Wnt hauv cov pob txha tsim ntawm cov nas uas muaj cov tshuaj fluoride, tab sis cov txheej txheem tseem tsis meej.Txoj kev tshawb fawb tam sim no tau txiav txim siab qhov kev koom tes ntawm Wnt / β-catenin signaling hauv fluoride-induced osteoblastic sib txawv.Siv thawj nas osteoblasts, peb pom tau tias fluoride ho txhawb osteoblasts proliferation thiab alkaline phosphate (ALP) qhia nrog rau cov mRNA qhia theem ntawm cov pob txha sib txawv cov cim, xws li hom I collagen (COL1A1), ALP thiab osteonectin.Peb ntxiv pom fluoride induced phosphorylations ntawm serine 473 ntawm Akt thiab serine 9 ntawm glycogen synthase kinase-3β (GSK3β), uas ua rau GSK-3β inhibition thiab tom qab lub nuclear tsub zuj zuj ntawm β-catenin, raws li qhia los ntawm Western blot thiab immunofluorescence tsom xam. .Tsis tas li ntawd, fluoride kuj tau ua rau kev qhia ntawm Wnt-targeted gene runt-related transcription factor 2 (Runx2).Qhov tseem ceeb, cov txiaj ntsig zoo ntawm fluoride ntawm ALP kev ua haujlwm thiab mRNA cov lus qhia ntawm COL1A1, ALP, osteonection thiab Runx2 raug tshem tawm los ntawm DKK-1, tus blocker ntawm Wnt / β-catenin receptor.Ua ke, cov kev tshawb pom no qhia tias fluoride txhawb kev sib txawv ntawm osteoblastic los ntawm Akt- thiab GSK-3β-dependent activation ntawm Wnt/β-catenin signaling pathway nyob rau hauv thawj nas osteoblasts.Peb qhov kev tshawb pom muab kev nkag siab tshiab rau hauv cov txheej txheem ntawm kev ua ntawm fluoride hauv osteoblastogenesis.
