Cov khoom

Peb tsim ib qho tshiab me me cuam tshuam RNA (siRNA) kev xa khoom siv lub ternary complex nrog polyethyleneimine (PEI) thiab γ-polyglutamic acid (γ-PGA), uas qhia tau hais tias ntsiag to thiab tsis muaj cytotoxicity.Lub binary complexes ntawm siRNA nrog PEI yog kwv yees li 73-102 nm hauv particle loj thiab 45-52 mV hauv ζ-muaj peev xwm.Cov nyhuv ntsiag to ntawm siRNA / PEI complexes nce nrog nce ntawm PEI, thiab siRNA / PEI complexes nrog tus nqi piv ntau dua 16 tau pom qhov tseem ceeb ntawm luciferase knockdown hauv nas kab mob qog noj ntshav ntawm tes tsis tu ncua qhia luciferase (Colon26 / Luc cells).Txawm li cas los xij, muaj zog cytotoxicity thiab ntshav agglutination tau pom nyob rau hauv siRNA / Lipofectamine complex thiab siRNA / PEI16 complex.Recharge cationic complexes nrog ib qho anionic compound tau tshaj tawm tias yog ib txoj hauv kev zoo rau kev kov yeej cov tshuaj toxicity.Peb thiaj li npaj ternary complexes ntawm siRNA nrog PEI (tus nqi piv 16) los ntawm qhov sib ntxiv ntawm γ-PGA kom txo tau cytotoxicity thiab xa siRNA.Raws li kev cia siab, cytotoxicity ntawm ternary complexes poob nrog kev nce ntawm γ-PGA cov ntsiab lus, uas txo cov ζ-muaj peev xwm ntawm cov complexes.Lub zog ntsiag to zoo piv rau siRNA / Lipofectamine complex tau pom nyob rau hauv ternary complexes nrog rau γ-PGA nrog rau anionic nto nqi.Kev koom ua ke siab ntawm ternary complexes rau hauv Colon26/Luc hlwb tau lees paub nrog fluorescence microcopy.Tom qab ua tiav qhov kev hloov pauv ntawm cov noob caj noob ces, lub peev xwm ntawm txoj hauv kev los daws qhov teeb meem ntawm lub tsev tu neeg endogenous, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), tau soj ntsuam hauv B16-F10 hlwb.Lub ternary complex (siRNA / PEI16 / γ-PGA12 complex) tau nthuav tawm GAPDH qhov cuam tshuam tseem ceeb.Yog li, peb tau tsim ib qho txiaj ntsig siRNA xa khoom.