Cov khoom

Qhov tshwm sim tsis txaus ntseeg ntawm qee cov chav kawm ntawm cov tshuaj tiv thaiv coagulants yog osteoporosis uas tej zaum yuav yog, yam tsawg kawg ntawm ib feem, cuam tshuam rau deranged mesenchymal qia cell (MSC) muaj nuj nqi.Lub hom phiaj ntawm txoj kev tshawb fawb tam sim no yog los sib piv cov txiaj ntsig ntawm fondaparinux (FDP), ib qho tshiab antithrombotic nrog ib txwm siv dav siv qis molecular hnyav heparin, tinzaparin (TZP) ntawm MSC proliferation thiab sib txawv.MSCs raug cais tawm ntawm cov pob txha trabecular ntawm 14 cov neeg mob raug mob los ntawm cov txheej txheem kev zom zaub mov collagenase thiab nthuav dav hauv cov txheej txheem kom txog rau thaum 3. Kev loj hlob thiab kev sib txawv ntawm MSCs rau chondrocytes thiab osteoblasts tau soj ntsuam nrog lossis tsis muaj qhov sib ntxiv ntawm FDP thiab TZP siv tus qauv hauv vitro. kev soj ntsuam thiab ntau yam ntawm cov tshuaj concentration.Flow cytometry tau siv rau MSC phenotyping.Nyob rau hauv pawg hnub nyoog kawm (17-74 xyoo) MSC zaus nyob rau hauv collagenase-tso cov feem yog 641/10(6) hlwb (ntau 110-2,158) thiab lawv cov yam ntxwv loj hlob yog ∼4 hnub / pej xeem ob npaug.Cov kab lis kev cai muaj tus qauv MSC phenotype (CD73+, CD105+, CD146+, CD106+, thiab CD166+).Cell proliferation tau soj ntsuam los ntawm ob qho tib si colony-forming unit-fibroblast (CFU-F) thiab colorimetric tetrazolium ntsev XTT kev soj ntsuam.Hauv ob qhov kev ntsuam xyuas, MSC proliferation tau inhibited los ntawm qhov sib ntxiv ntawm TZP, tshwj xeeb tshaj yog nyob rau hauv siab concentrations.Hauv qhov sib piv, FDP tsis cuam tshuam rau MSC kev loj hlob.Osteogenic sib txawv thiab chondrogenic sib txawv tsis cuam tshuam los ntawm kev sib ntxiv ntawm TZP lossis FDP.Txawm hais tias MSC kev loj hlob, tab sis tsis sib txawv, muaj kev cuam tshuam tsis zoo los ntawm TZP, tsis muaj pov thawj rau qhov tsis zoo ntawm FDP hauv cov qauv hauv vitro uas sib cav zoo rau nws siv hauv qhov chaw orthopedic.