Cov khoom

Cov nyhuv ntawm acidosis, biochemical pom ntawm qog microenvironment, ntawm kev mob qog noj ntshav thiab metastasis yog qhov nyuaj.Ob leeg pro- thiab anti-tumorigenic teebmeem ntawm acidosis tau raug tshaj tawm thiab cov acidic microenvironment tau siv rau kev xa cov tshuaj, tshuaj ntsuam xyuas, thiab kev tsim cov caj ces rau hauv cov qog.Hauv txoj kev tshawb no peb tshawb xyuas qhov sib kis thiab focal adhesion ntawm B16F10 melanoma hlwb uas yog genetic engineered los overexpress lub pH-sensing G protein-coupled receptor GPR4.Los ntawm kev siv cov ntaub ntawv sib txuas ntawm tes peb pom tias GPR4 overexpression ncua kev sib kis ntawm tes thiab hloov kho qhov chaw nyob ntawm qhov chaw ntawm qhov chaw ntawm qhov chaw focal adhesion complex, xws li localization ntawm phosphorylated focal adhesion kinase (FAK) thiab paxillin, ntawm acidic pH.Lub peev xwm G-protein thiab downstream signaling txoj hauv kev uas yog lub luag haujlwm rau cov teebmeem no kuj tau tshawb xyuas.Los ntawm kev siv Rho inhibitor CT04 (C3 transferase), Rho-associated kinase (ROCK) inhibitors Y27632 thiab thiazovivin, myosin light chain kinase (MLCK) inhibitor staurosporine lossis G12/13 inhibitory tsim, cell kis tau rov qab thaum qhov inhibition thiab Kev ua kom txoj hauv kev Gq thiab Gs tsis muaj txiaj ntsig.Ua ke peb cov txiaj ntsig qhia tau hais tias dhau los ntawm G12/13 / Rho signaling pathway GPR4 modulates focal adhesion dynamics thiab txo cov cell kis thiab daim nyias nyias.