dCTP, 2'-Deoxycytidine-5'-triphosphoric acid disodium ntsev Cas: 102783-51-7 Dawb hmoov
| Catalog Number | xwm 90584 |
| Khoom npe | dCTP, 2'-Deoxycytidine-5'-triphosphoric acid disodium ntsev |
| CAS | 951-78-0 : kuv |
| Molecular Formula | C9H12N2O5 |
| Molecular Luj | 228.20 Nws |
| Cov ntaub ntawv khaws cia | -20 ° C |
| Harmonized tariff Code | 293499 0 |
Khoom Specification
| Qhov tshwm sim | Dawb hmoov |
| Assay | 99% |
Eukaryotic hlwb muaj qhov sib npaug me me ntawm feeb ntawm plaub deoxyribonucleoside triphosphates (dNTPs), txaus rau ob peb feeb ntawm DNA replication.Ob qho tib si qhov tsis muaj peev xwm thiab qhov seem ntawm ib qho dNTP yuav ua rau muaj kev hloov pauv ntau ntxiv, kho DNA tsis raug lossis mitochondrial DNA depletion.dNTPs feem ntau yog ntsuas los ntawm qhov kev ntsuam xyuas enzymatic uas suav nrog cov xov tooj cua dATP (los yog cov xov tooj cua dTTP hauv qhov kev ntsuam xyuas rau dATP) rau hauv cov hluavtaws oligonucleotides los ntawm DNA polymerase yog proportional rau qhov concentration ntawm cov tsis paub dNTP.Peb pom tias Klenow DNA polymerase feem ntau siv los hloov cov ribonucleotide coj mus rau qhov tsis paub dNTP ua rau qee qhov kev kwv yees loj ntawm dNTPs.Tam sim no peb piav qhia txog cov xwm txheej ntsuas rau txhua dNTP uas zam kev koom ua ke ntawm ribonucleotide.Rau dTTP thiab dATP kev soj ntsuam nws txaus kom txo qis cov ntsiab lus ntawm Klenow enzyme thiab cov ntawv sau npe dATP (lossis dTTP);rau dCTP thiab dGTP peb yuav tsum tau hloov t nws Klenow enzyme nrog rau Taq DNA polymerase los yog Thermo Sequenase.Peb xav tias nyob rau hauv qee cov ntawv tshaj tawm ua ntej, kev koom ua ke ntawm ribonucleotide tuaj yeem ua rau muaj txiaj ntsig zoo rau dGTP thiab dCTP.
