Chymotrypsinogen A CAS: 9035-75-0 Dawb crystaline hmoov
| Catalog Number | xwm 90376 |
| Khoom npe | Chymotrypsinogen A |
| CAS | 9 035-75-0 |
| Molecular Formula | C47H58FeN4O4S2 |
| Molecular Luj | 862.96 ib |
| Cov ntaub ntawv khaws cia | -20 ° C |
Khoom Specification
| Assay | 99% |
| Qhov tshwm sim | Dawb crystaline hmoov |
Adsorption ntawm cov proteins rau cov khoom sib txuas ntawm cov kua dej feem ntau pom pom los txhawb kev tsim cov dej sib xyaw ua ke thiab loj dua, pom tau, thiab pom tau, tab sis cov theem tseem ceeb hauv cov txheej txheem no feem ntau nyuaj rau kev soj ntsuam ncaj qha.Aggregation mediated by adsorption to water-silicon oxide (SiOx) interfaces, akin to hydrated glass surfaces, was characterized as a function of pH and ionic zog for alpha-chymotrypsinogen (aCgn) and for a monoclonal antibody (IgG1).Ib lub xovtooj ntawm tes tso cai rau neutron reflectivity rau cov txheej txheem protein adsorbed los ntxuav SiOx nto, nrog rau tom qab ua tiav "yuam" cov kauj ruam.Aggregates rov qab los rau hauv kev daws teeb meem tom qab maj mam "ntxuav" qhov saum npoo tau pom los ntawm neutron scattering, microscopy, thiab fluorescence spectroscopy.IgG1 molecules taw qhia feem ntau "pav" tawm tsam SiOx nto, nrog cov txheej txheem protein ntau desorbed rau qhov tsawg, hos lub diffuse overlayer tau yooj yim yaug tawm.aCgn molecules tau resistant rau desorption thaum lawv ib tug ppeared yuav tsum unfolded ntawm lub interface, tab sis lwm yam yooj yim tshem tawm.Rau cov xwm txheej uas muaj kev sib khi muaj zog, cov protein uas ua rau desorb yog sib xyaw ntawm monomer thiab me me ntawm HMW aggregates (rau aCgn) lossis cov khoom tsis pom (rau IgG1).Kev hloov pauv hauv adsorption thiab / lossis nthuav tawm nrog pH qhia tias kev sib cuam tshuam electrostatic tseem ceeb hauv txhua kis.
