Cov khoom

Kev soj ntsuam ntawm pharmacodynamics ntxiv rau cov tshuaj pharmacokinetics yog ib qho ntawm cov tswv yim los kho tus kheej ntawm mycophenolate mofetil therapy.Lub hom phiaj ntawm txoj kev tshawb no yog los tsim cov rhiab heev kua chromatography-tandem huab hwm coj spectrometry (LC-MS / MS) txoj kev rau kev ntsuam xyuas ntawm lub pharmacokinetics thiab pharmacodynamics ntawm mycophenolic acid (MPA).Concentrations ntawm mycophenolic acid glucuronide (MPAG), mycophenolic acid acyl-glucuronide, as well as unbound MPA thiab MPAG, tau txiav txim, thiab inosine-5'-monophosphate dehydrogenase kev ua tau xam los ntawm kev ntsuas qhov concentration ntawm cov khoom xanhosine-5'-monophosphate (XMP) ) thiab intracellular adenosine-5'-monophosphate tom qab incubation ntawm peripheral ntshav mononuclear cell (PBMC) lysates.Ib kem tsis muaj hlau tsis muaj Mastro (TM) thiab ob tus qauv gradient tau siv los txhim kho qhov kev txwv ntau ntawm XMP rau 0.1 μM.Nyob rau hauv kev kho mob MPA concentration ntau, lub linearity ntawm lub calibration nkhaus, inter- thiab intra-hnub precision thiab raug txaus siab rau qhov relevant US Food and Drug Administration cov lus qhia.MPA concentrations nyob rau hauv hematopoietic stem cell transplant (HSCT) cov neeg mob txiav txim los ntawm lub enzyme assay thiab tam sim no LC-MS / MS txoj kev qhia ib tug zoo correlation (r(2) = 0.95, p <0.001).Nyob rau hauv txoj kev tshawb no, peb tshaj tawm rhiab thiab siv tau LC-MS/MS txoj kev los ntsuam xyuas cov tshuaj pharmacokinetics thiab pharmacodynamics ntawm MPA, uas yog txaus rhiab heev rau ntsuam xyuas cov me me ntawm PBMC lysates sau sai tom qab HSCT.