Cov khoom

Ib daim ntawv lo-dawb electrochemiluminescence (ECL) immunosensor rau ultrasensitive thiab sai nrhiav tau ntawm marine pathogenic kab mob Vibrio parahaemolyticus (VP) nyob rau hauv dej hiav txwv thiab nqaij nruab deg tau tsim raws li multi-functionalized graphene oxide, uas tau npaj nrog N-(4-aminobutyl)-N -ethylisoluminol (ABEI) thiab VP antibody (anti-VP) ib txhij immobilized rau saum npoo ntawm hlau nplaum graphene oxide (nanoFe3O4@GO).ABEI thiab tiv thaiv VP ua raws li electrochemiluminophore thiab cov cuab yeej ntes rau VP.Zoo conductivity thiab ob-dimensional qauv ntawm nanoFe3O4 @ GO enabled tag nrho cov ABEI, immobilized ntawm GO, electrochemically active thiab yog li txhim kho qhov kev tshawb pom rhiab heev.Nyob rau hauv cov xwm txheej zoo, ECL kev siv zog txo qis nrog kev nce logarithmic concentrations ntawm VP nyob rau hauv thaj tsam ntawm 10-10 (8) CFU / mL, nrog rau kev tshawb pom txwv ntawm 5CFU / mL rau dej hiav txwv thiab 5CFU / g rau nqaij nruab deg.Qhov no ECL immunosensor tau pom muaj qhov tshwj xeeb, ruaj khov thiab rov tsim dua tshiab rau kev tshawb pom VP.Tsis tas li ntawd, ECL immunosensor tau siv los txiav txim siab qhov concentration ntawm VP hauv cov dej hiav txwv thiab cov nqaij nruab deg sai, nrog kev rov qab los ntawm 94.4-112.0% thiab RSD 4.1-11.7%.Yog li, tus tsim immunosensor qhia tau hais tias muaj kev cia siab zoo rau kev siv tswv yim.