6-Chloropurine CAS: 87-42-3 Lub teeb daj hmoov
Catalog Number | xwm 90547 |
Khoom npe | 6-Chloropurine |
CAS | 87-42-3 : kuv |
Molecular Formula | C5H3ClN4 |
Molecular Luj | 154.557 Nws |
Cov ntaub ntawv khaws cia | 2 txog 8 ° C |
Harmonized tariff Code | 293390090 ib |
Khoom Specification
Qhov tshwm sim | Lub teeb daj hmoov |
Assay | 99% |
Peb tsis ntev los no tau tshaj tawm tias pyrene nucleotide yog qhov zoo dua tso rau sab nraud ntawm qhov chaw abasic, 3'-T ntawm thymine dimer, thiab feem ntau cov hauv paus tsis puas los ntawm cov poov xab DNA polymerase eta (pol eta).Vim hais tias pyrene yog ib tug nonpolar molecule uas tsis muaj H-bonding muaj peev xwm, qhov txawv txav tau zoo ntawm dPMP insertion yog ascribed rau nws superior base stacking peev xwm, thiab underscore qhov tseem ceeb ntawm lub hauv paus stacking nyob rau hauv kev xaiv ntawm nucleotides los ntawm pol eta.Txhawm rau tshawb xyuas lub luag haujlwm ntawm H-bonding thiab lub hauv paus khub geometry hauv kev xaiv cov nucleotides los ntawm pol eta, peb txiav txim siab qhov kev ua tau zoo ntawm lub hauv paus-hloov nucleotides 2,6-diaminopurine, 2-aminopurine, 6-chloropurine, thiab inosine uas yuav ua kom muaj tus lej sib txawv ntawm H-daim ntawv cog lus nrog lub hauv paus qauv nyob ntawm lub hauv paus khub geometry.Watson-Crick puag khub zoo li ua lub luag haujlwm tseem ceeb hauv kev xaiv cov nucleotide analogues rau kev ntxig rov qab C thiab T raws li pom los ntawm kev txo qis hauv cov txheeb ze kev cuam tshuam nrog kev txo qis ntawm Watson-Crick H-bonds thiab nce hauv tus naj npawb ntawm tus neeg pub dawb thiab tus neeg txais txais kev sib cuam tshuam.Kev xaiv ntawm nucleotide insertion yog ntau dua qhov sib txawv ntawm 5'-T tshaj li 3'-T ntawm thymine dimer, raws li kev ua haujlwm dhau los qhia tias 5'-T yog tuav nruj dua li 3'-T.Tsis tas li ntawd, kev nkag ntawm A opposite ob qho tib si Ts ntawm lub dimer zoo li yuav tsum tau kho los ntawm Watson-Crick puag khub thiab tsis yog los ntawm Hoogsteen puag ua ke raws li qhov yuav luag zoo tib yam kev nkag siab zoo ntawm A thiab 7-deaza-A, tom kawg uas tsis muaj H- Kev sib txuas muaj peev xwm ntawm N7.Cov txheeb ze ua tau zoo rau kev nkag ntawm nucleotides uas tuaj yeem tsim Watson-Crick puag khub sib luag rau cov Klenow fragment, thaum Klenow fragment muaj kev ntxub ntxaug tawm tsam qhov tsis sib haum xeeb, raws li nws qhov kev xaiv ntau dua.Cov txiaj ntsig no qhia txog qhov tseem ceeb ntawm H-bonding thiab Watson-Crick puag khub geometry hauv kev xaiv ntawm nucleotides los ntawm ob qho tib si pol eta thiab Klen ow fragment, thiab lub luag haujlwm tsawg dua ntawm kev xaiv cov duab hauv kev ntxig los ntawm pol eta vim nws qhib thiab tsawg dua. txwv qhov chaw active.