Cov khoom

Polycations yog tshawb nrhiav los ua tus nqa khoom kom xa cov tshuaj nucleic acids.Polycations yog conventionally pharmacological inert nrog ib qho kev ua haujlwm ntawm kev xa cov khoom kho mob.Txoj kev tshawb no qhia txog kev sib txuas ntawm tus kheej-immolative polycation (DSS-BEN) raws li cov tshuaj polyamine analogue N(1), N(11)-bisethylnorspermine (BENSpm).Lub polycation tau tsim los ua haujlwm dual ua tus xa cov noob caj noob ces thiab cov tshuaj tiv thaiv kab mob dysregulated polyamine metabolism hauv qog noj ntshav.Siv kev sib xyaw ua ke ntawm NMR thiab HPLC, peb paub tseeb tias tus kheej-immolative polycation tau dhau los ntawm kev degradation ntawm lub cev mus rau niam txiv cov tshuaj BENSpm.Qhov tso tawm BENSpm depletes cellular theem ntawm spermidine thiab spermine thiab upregulates polyamine catabolic enzymes spermine / spermidine N(1)-acetyltransferase (SSAT) thiab spermine oxidase (SMO).Cov synthesized polycations tsim polyplexes nrog DNA thiab pab txhawb kev hloov pauv tau zoo.Ua kom zoo dua ntawm lub peev xwm ntawm BENSpm los ua kom mob qog noj ntshav rau TNFα-induced apoptos yog, peb qhia tias DSS-BEN txhim kho cov cell tua kev ua haujlwm ntawm TNFα gene therapy.Cov ntaub ntawv tshawb fawb tau lees paub DSS-BEN raws li kev xa tawm ob lub luag haujlwm uas tuaj yeem xa cov noob tshuaj kho mob thiab txhim kho cov txiaj ntsig ntawm kev kho noob raws li qhov tshwm sim ntawm kev degradation intracellular ntawm DSS-BEN rau BENSpm thiab cov txiaj ntsig tom qab ntawm BENSpm ntawm dysregulated polyamine. metabolism hauv mob qog noj ntshav.