Cov kab mob colonic, piv txwv los ntawm Bacteroides thetaiotaomicron, ua lub luag haujlwm tseem ceeb hauv kev saib xyuas tib neeg kev noj qab haus huv los ntawm kev siv cov tsev neeg loj ntawm glycoside hydrolases (GHs) los siv cov khoom noj khoom haus polysaccharides thiab host glycans ua cov as-ham.Xws li GH tsev neeg nthuav dav yog ua piv txwv los ntawm 23 tsev neeg GH92 glycosidases encoded los ntawm B. thetaiotaomicron genome.Ntawm no peb qhia tau hais tias cov no yog alpha-mannosidases uas ua los ntawm ib qho kev txav chaw los siv tus tswv N-glycans.Cov qauv peb-dimensional ntawm ob GH92 mannosidases txhais ib tsev neeg ntawm ob lub npe proteins uas lub chaw catalytic nyob ntawm qhov chaw sib txuas, muab cov kua qaub (glutamate) thiab lub hauv paus (aspartate) kev pab rau hydrolysis hauv Ca(2+)- kev vam meej.Cov qauv peb-dimensional ntawm GH92s nyob rau hauv complex nrog inhibitors muab kev nkag siab rau qhov tshwj xeeb, mechanism thiab conformational itinerary ntawm catalysis.Ca(2+) plays lub luag haujlwm tseem ceeb hauv kev pab cuam tshuam cov mannoside kom deb ntawm nws hauv av-xeev (4)C(1) lub rooj zaum hloov mus rau lub xeev hloov
