Cov khoom

1.Peb tau kawm txog cov teebmeem inhibitory ntawm isorhamnetin ntawm nceb tyrosinase los ntawm inhibition kinetics thiab kev suav simulation.Isorhamnetin reversibly inhibited tyrosinase hauv hom sib xyaw ntawm Ki = 0.235 ± 0.013 mM.Kev ntsuas ntawm intrinsic thiab 1-anilinonaphthalene-8-sulfonate (ANS)-binding fluorescence pom tias isorhamnetin tsis ua rau muaj kev hloov pauv loj hauv cov qauv tertiary ntawm tyrosinase.Txhawm rau kom nkag siab txog cov txheej txheem inactivation, cov kinetics tau xam los ntawm kev ntsuas lub sij hawm-interval thiab cov tshuaj tiv thaiv tsis tu ncua.Cov txiaj ntsig tau qhia tias kev tsis ua haujlwm tshwm sim los ntawm isorhamnetin yog thawj qhov kev txiav txim nrog cov txheej txheem biphasic.Kom tau txais kev pom ntxiv, peb simulated docking ntawm tyrosinase thiab isorhamnetin.Kev simulation tau ua tiav (kev khi lub zog rau Dock6.3: -32.58 kcal / mol, rau AutoDock4.2: -5.66 kcal / mol, thiab rau Fred2.2: -48.86 kcal / mol), qhia tias isorhamnetin cuam tshuam nrog ntau qhov seem, xws li Raws li HIS244 thiab MET280.Qhov no lub tswv yim ntawm predic ting tyrosinase kev sib cuam tshuam nyob rau hauv ua ke nrog kinetics raws li ib tug flavanone compound yuav ua pov thawj pab tau nyob rau hauv kev tshuaj ntsuam rau tej yam ntuj tso tyrosinase inhibitors.

2.Acid unfolding pathway of conalbumin (CA), monomeric glycoprotein los ntawm hen qe dawb, tau raug tshawb xyuas siv deb- thiab ze-UV CD spectroscopy, intrinsic fluorescence emission, extrinsic fluorescence sojntsuam 1-anilino-8-napthalene sulfonate (ANS) thiab dynamic light scattering (DLS).Peb soj ntsuam pH-raws li kev hloov pauv hauv cov qauv theem nrab thiab theem nrab ntawm CA.Nws muaj cov qauv zoo li α-helical theem nrab ntawm pH 4.0 tab sis poob qauv ntawm pH 3.0.Lub CA muaj tshwj xeeb tshaj yog nyob rau hauv pre-molten globule xeev thiab molten globule xeev nyob rau hauv kev daws ntawm pH 4.0 thiab pH 3.0, feem.Cov nyhuv ntawm pH ntawm kev sib haum xeeb thiab qhov ntsuas kub ntawm CA cov ntsiab lus rau nws qhov kub tsis kam ntawm pH nruab nrab.DLS cov txiaj ntsig tau pom tias MG lub xeev muaj nyob rau hauv daim ntawv cog lus hauv cov tshuaj aqueous nrog hydrodynamic radii ntawm 4.7 nm.Quenching ntawm tryptophan fluorescence los ntawm acrylamide ntxiv tau lees paub qhov tsub zuj zuj ntawm lub xeev nruab nrab, ib nrab nthuav tawm, nyob rau hauv-nruab nrab ntawm haiv neeg thiab cov xeev unfolded.