Cov khoom

Submillimolar concentrations ntawm cytotoxic bile acids (BAs) induce cell tuag ntawm apoptosis.Ntawm qhov tod tes, ob peb cytoprotective BAs tau pom tias tiv thaiv apoptosis nyob rau tib qhov concentration ntau.Txawm li cas los xij, cov txheej txheem uas BAs ua rau cov teeb meem tsis sib haum xeeb tseem tsis meej.Txoj kev tshawb no yog tsom los txiav txim siab seb puas muaj cytotoxic thiab cytoprotective BAs, ntawm physiologically active concentrations, muaj peev xwm hloov kho lub biophysical zog ntawm lipid daim nyias nyias, muaj peev xwm txhais tau rau hauv kev hloov pauv hauv apoptotic pib ntawm cov hlwb.Kev khi ntawm BAs rau daim nyias nyias tau soj ntsuam los ntawm kev hloov pauv ntawm fluorescence tsis haum ntawm cov khoom tsim nyog BAs.Cov derivatives no tau muab faib nrog ntau dua affinity rau cov kua disordered dua li cov cholesterol-enriched kua tau txiav txim domains.Unlabeled BAs kuj tau pom tias muaj qhov chaw nyob sab nraud thaum muaj kev cuam tshuam nrog lipid membrane.Tsis tas li ntawd, kev sib cuam tshuam ntawm cytotoxic BAs nrog daim nyias nyias ua rau cov membrane nthuav dav, raws li cov ntaub ntawv FRET.Ntxiv mus, nws tau pom tias cytotoxic BAs tuaj yeem cuam tshuam qhov kev txiav txim ntawm daim nyias nyias los ntawm cov roj (cholesterol) ntawm lub cev muaj zog ntawm BA, qhov cuam tshuam tsis cuam tshuam nrog kev tshem tawm cov roj cholesterol.Ntawm qhov tod tes, cytoprotective bile acids tsis muaj kev cuam tshuam rau daim nyias nyias.Nws tau txiav txim siab tias, muab cov txiaj ntsig pom ntawm daim nyias nyias rigidity, kev ua haujlwm apoptotic ntawm cytotoxic BAs tuaj yeem cuam tshuam nrog kev hloov pauv hauv plasma membrane lub koom haum (xws li kev hloov pauv ntawm lipid domains) lossis nrog kev nce hauv mitochondrial membrane affinity rau apoptotic proteins.