Ob isomeric bicyclo[4.1.0] heptane analogues ntawm glycosidase inhibitor galacto-validamine, (1R *, 2S, 3S, 4S, 5S, 6S *)-5-amino-1-(hydroxymethyl)bicyclo[4.1.0] heptane -2,3,4-triol, tau raug synthesized hauv 13 kauj ruam ntawm 2,3,4,6-tetra-O-benzyl-D-galactose.Cov kev ua haujlwm inhibitory ntawm ob qhov kev txwv tsis pub muaj amines, thiab lawv cov acetamides sib raug, raug ntsuas tawm tsam kev lag luam alpha-galactosidase enzymes los ntawm kas fes taum thiab E. coli.Cov kev ua ntawm glycosyl hydrolase tsev neeg GH27 enzyme (coffee taum) tau sib tw los ntawm 1R,6S-amine (7), kev sib txuas lus uas tau pom los ntawm K (i) tus nqi ntawm 0.541 microM.GH36 E. coli alpha-galactosidase tau nthuav tawm ntau qhov kev sib txuam tsis muaj zog nrog 1R,6S-amine (IC(50) = 80 microM).Lub diastereomeric 1S,6R-amine (9) khi tsis muaj zog rau ob qho tib si galactosidases, (coffee taum, IC(50) = 286 microM) thiab (E. coli, IC(50) = 2.46 mM).