Cov khoom

Ib qho EOF counter-balanced ITP ciam teb tau siv los tso cov anions los ntawm cov qauv coj mus kuaj thaum lub sij hawm txuas ntxiv electrokinetic ntawm cov qauv.Hauv ib qho polystyrenesulfonate / poly (diallyldimethylammonium chloride) polyelectrolyte coated capillary, lub sij hawm uas ITP ciam teb tawm ntawm lub capillary tuaj yeem ua kom ntev los ntawm kev ntsuas qhov txav ntawm ciam teb nrog EOF.Siv bis-tris-propane electrolyte, ITP ciam teb raug tshem tawm ntawm lub capillary hauv 7min, thaum siv triethanolamine ITP ciam teb tseem nyob ntawm 30% ntawm cov hlab ntsha tom qab 2h ntawm kev txhaj tshuaj.Siv cov tshuab no, qhov rhiab heev ntawm kev sib xyaw ntawm cov organic acids yooj yim hauv 100mM Cl (-) tau txhim kho los ntawm 700-800-fold siv bis-tris-propane nrog rau tag nrho-capillary txhaj ntawm cov qauv thiab 5min ntawm electrokinetic txhaj ntawm +28kV , thiab 1100-1300-fold siv triethanolamine thiab 60min ntawm electrokinetic txhaj nyob rau hauv tib yam mob.Lub peev xwm ntawm txoj kev yuav siv tau rau cov qauv coj mus kuaj siab tau pom los ntawm stacking tag nrho cov capillary uas muaj cov zis spiked nrog naphthalenedisulfonic acid, nrog rau kev txwv ntawm kev tshawb pom 450 npaug qis dua cov uas ua tau nrog ib txwm hydrodynamic txhaj.