Cov khoom

Cysteine ​​(Cys) plays lub luag haujlwm tseem ceeb hauv ntau cov txheej txheem physiological ntawm eukaryotic hlwb thiab nws qhov kev kuaj pom hauv cov hlwb yog qhov tseem ceeb.Txawm li cas los xij, glutathione (GSH), homocysteine ​​​​, N-acetyl-L-cysteine ​​​​thiab lwm yam thiols cuam tshuam rau kev kuaj pom Cys.Tshwj xeeb, GSH cuam tshuam nrog kev tshawb pom ntawm cellular Cys (30-200 μM) vim nws qhov siab intracellular concentration (1-10 mM).Hauv kev ua haujlwm no, kev sojntsuam tawm ntawm fluorescent (HOTA) rau kev kuaj pom ntawm Cys tau nthuav tawm.Qhov kev sojntsuam no muaj ob qho tib si zoo heev rhiab heev thiab txaus siab xaiv rau cellular Cys nrhiav pom: nrog rau qhov sib ntxiv ntawm 200 μM Cys, fluorescence siv ntawm qhov kev sojntsuam (10 μM) txhim kho 117-fold thiab qhov kev kuaj pom tau suav tias yog 13.47 μM, uas yog qis dua. tshaj qhov cellular Cys concentration;Qhov kev sojntsuam kuj tau kuaj pom 30–200 μM cysteine ​​ntau dua 1-10 mM glutathione.Yog li ntawd, kev sim duab ntawm tes tau ua nrog kev sojntsuam HOTA.Tsis tas li ntawd, cov txiaj ntsig ntawm thiol-blocki ng thiab GSH synthesis inhibiting thwmsim tau lees paub tias cov emission intracellular feem ntau yog los ntawm kev sib cuam tshuam ntawm Cys thiab HOTA.