Streptomycin sulfate CAS: 3810-74-0 Dawb hmoov
| Catalog Number | xwm 90361 |
| Khoom npe | Streptomycin sulfate |
| CAS | 3810-74-0 : kuv |
| Molecular Formula | (C21H39N7O12) 2 · 3H2SO4 |
| Molecular Luj | 1457.39 Nws |
| Cov ntaub ntawv khaws cia | 2 txog 8 ° C |
| Harmonized tariff Code | 29412080 ib |
Khoom Specification
| Qib | USP36/EP8.0 |
| pH | 4.5-7.0 Nws |
| Poob rau ziab | ≤ 7.0% |
| Assay | ≥720IU/MG |
| Sulfate | 18.0 ~ 21.5% |
| Qhov tshwm sim | Dawb los yog yuav luag dawb hmoov, tsis tshua muaj soluble hauv dej, yuav luag insoluble hauv ethanol. |
| Methanol | ≥0.3% |
| Streptomycin B | ≤ 3.0% |
| Colorimetric Test | ≥90.0% |
| Sulfuric Acid | ≤ 1.0% |
| Concentrated sodium sulfite cov ntsiab lus | ≤ 0.4% |
Txawm hais tias etiology ntawm intervertebral disc degeneration tsis to taub, ib txoj hauv kev los tiv thaiv cov txheej txheem no yuav yog inhibit apoptosis.Nyob rau hauv txoj kev tshawb no tam sim no, cov tshuaj tiv thaiv apoptotic ntawm carboxymethylated chitosan (CMCS) nyob rau hauv nucleus pulposus (NP) hlwb raug soj ntsuam nrog lub hom phiaj los txhim kho disc cell ciaj sia taus.Rat NP hlwb raug cais tawm thiab coj mus rau hauv vitro, thiab hydrogen peroxide (H2O2) tau siv los tsim NP cell apoptosis qauv.Cell viability raug soj ntsuam nrog cov khoom siv suav cell-8 assay.Qhov piv ntawm apoptotic hlwb raug soj ntsuam los ntawm annexin V-fluorescein isothiocyanate (FITC) thiab propidium iodide (PI) ob chav staining tsom xam, thiab morphology tau soj ntsuam los ntawm Hoechst 33342 staining.Lub mitochondrial membrane muaj peev xwm ntawm NP hlwb raug soj ntsuam los ntawm rhodamine 123 fluorescence staining.Reverse transcription (RT)-quantitative polymerase chain reaction (qPCR) tau ua los ntsuas mRNA qib ntawm inducible nitric oxide synthase (iNOS), caspase-3, B-cell lymphoma (Bcl)-2, hom II collagen thiab aggrecan.Western blot tsom xam tau ua kom pom cov qib protein ntawm iNOS thiab Bcl-2.Lub annexin V-FITC/PI thiab Hoechst 33342 staining tshwm sim qhia tias CMCS muaj peev xwm tiv thaiv NP hlwb los ntawm apoptosis nyob rau hauv ib koob tshuaj.Rhodamine 123 staining qhia meej tias CMCS txo qhov kev puas tsuaj ntawm mitochondrial membrane muaj peev xwm hauv H2O2-kho NP hlwb.Txo cov caspase-3 thiab nce Bcl-2 kev ua haujlwm tau tshawb pom hauv CMCS-kho NP hlwb los ntawm RT-qPCR thiab western blot tsom xam.CMCS kuj txhawb nqa kev loj hlob thiab tso tawm ntawm hom II collagen thiab aggrecan hauv H2O2-kho NP hlwb.CMCS tau qhia tias muaj txiaj ntsig zoo hauv kev tiv thaiv apoptotic cell tuag hauv vitro, ua kom pom cov txiaj ntsig zoo ntawm qhov kev kho mob no hauv kev tswj cov disc degeneration.
