Cov khoom

Txoj kev tshawb no piav qhia txog ib txoj hauv kev yooj yim thiab siv tau zoo siv cov kua dej chromatography (HPLC) ua ke rau kev kuaj pom fluorescence rau kev txiav txim siab ntawm cov kev ntsuas kinetic ntawm glutamate uptake hauv paj hlwb.Retinal cov ntaub so ntswg tau txais los ntawm 7-hnub menyuam qaib tau incubated nrog paub cov concentrations ntawm glutamate (50-2000 μM) rau 10 min, thiab cov theem ntawm o-phtaldehyde (OPA)-derivatized neurotransmitter nyob rau hauv incubation nruab nrab tau ntsuas.Los ntawm kev ntsuam xyuas qhov sib txawv ntawm qhov pib thiab qhov kawg ntawm qhov siab ntawm glutamate nyob rau hauv nruab nrab, lub saturable uptake mechanism yog tus yam ntxwv (K(m) = 8.2 thiab V (max) = 9.8 nmol / mg protein / min).Qhov kev ntsuas no feem ntau yog sodium- thiab qhov kub thiab txias, txhawb nqa tias cov txheej txheem rau cov concentration decrements yog tiag tiag uptake los ntawm high-affinity transporters.Ntxiv rau qhov no, peb cov txiaj ntsig kuj tau pom tias zinc chloride (ib qho inhibitor ntawm glutamate / aspartate transporters) evoked ib qho concentration-dependant txo qis hauv glutama te uptake, qhia qhov tshwj xeeb ntawm peb cov txheej txheem.Zuag qhia tag nrho, txoj haujlwm tam sim no qhia txog lwm txoj hauv kev los ntsuas glutamate uptake hauv paj hlwb siv HPLC.Txoj hauv kev no tuaj yeem yog ib qho cuab yeej tseem ceeb rau cov kev tshawb fawb cuam tshuam rau tus cwj pwm ntawm cov kev hloov pauv hauv feeb hauv glutamate thauj cuam tshuam nrog kev raug mob hauv nruab nrab paj hlwb.